Fig. 1

Overview of the four different delivery methods used to administer viral vector to the mouse respiratory tract. a Intranasal technique. Mice were anesthetized with isoflurane and the viral vector administered drop wise over the nares allowing for passive inhalation of the substance. b Modified-intranasal technique. Mice were anesthetized with isoflurane and the viral vector administered drop wise over the nares. The mouth is covered during administration to promote inhalation through the nose and subsequent distribution of the vector throughout the lower respiratory tract as well as to prevent swallowing of the administered substance. c Intubation technique. Mice were anesthetized with isoflurane in oxygen and a 22-gauge catheter placed into the trachea. The viral vector was administered to the airways through the catheter. d Intratracheal injection technique. Mice were anesthetized with isoflurane and the viral vector injected directly into the trachea following surgical visualization