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Table 2 Comparison of expected uidA and yaiO PCR products by Primer-BLAST analysis

From: Improved detection of Escherichia coli and coliform bacteria by multiplex PCR

Organism

Total number of hits

yaiO vs. uidA

yaiO +

uidA +

yaiO + uidA -

yaiO + uidA+

yaiO – uidA +

Escherichia coli

83

91

31

52

39

Escherichia sp.

1

2

1

 

2

Citrobacter rodentium

 

1

  

1

Shigella boydii

1

2

 

1

1

Shigella flexneri

 

6

  

6

Shigella sonnei

2

2

 

2

 
 

87

104

   
  1. The number of potential target sequences yielding positive in silico PCR amplification (N = 136) with yaiO or uidA primers (Table 1) are grouped by species or genus. The genomes that produce single and double amplicons are compared on the right
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BMC Biotechnology

ISSN: 1472-6750

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