Does size matter? Separations on guard columns for fast sample analysis applied to bioenergy research

BMC Biotechnology

Table 1 Calibration results for each compound

Compound	Detection	Linear quantification range	Regression coefficient
Ethanol	RID	0.01 − 50 mg/mL	0.9995
Acetone	RID	0.01 − 50 mg/mL	0.9996
	UV 265 nm	0.01 − 50 mg/mL	0.9999
	UV 285 nm	0.02 − 50 mg/mL	0.9995
iso-Butanol	RID	0.005 − 50 mg/mL	0.9999
n-Butanol	RID	0.005 − 50 mg/mL	0.9997
5-HMF	RID	0.005 − 50 mg/mL	0.9999
	UV 280 nm	0.00005 − 0.25 mg/mL	0.9999
Furfural	RID	0.005 − 50 mg/mL	0.9995
	UV 280 nm	0.00005 − 0.25 mg/mL	0.9999
Glucose	RID	0.005 − 20 mg/mL	0.9999
Xylose	RID	0.005 − 20 mg/mL	0.9999

Chromatography conditions: 50 mm × 7.8 mm RFQ Fast Acid H⁺ kept at 30 °C, flow rate: 1.0 ml/min of 5 mM sulfuric acid, 20 μL injection volume; Lower limit of the calibration range was determined as the concentration level with a relative standard deviation (RSD) <10 % (n = 5)

ISSN: 1472-6750