Fig. 6

Chromatogram of the supernatant from Saccharomyces cerevisiae SA-1 strain fermentation supplemented with 25 % of pH adjusted hydrolysate 3 analyzed with the 50 mm guard column showing glucose (peak 1), xylose (peak 2) and ethanol (peak 3) elution after (a) 42 h, (b) 50 h and (c) 67 h, and xylose and ethanol concentration profile of the supernatant between 0 h and 62 h from a S. cerevisiae SA-1 strain fermentation supplemented with (d) 25 % of pervaporation detoxified hydrolysate 3 and (e) 25 % of pH adjusted hydrolysate 3, analyzed with the 50 mm guard column and 300 mm column. HPLC parameters: column temperature 55 °C, mobile phase 5 mM sulfuric acid, flow rate 1.0 mL/min (0.6 mL/min for 300 mm column), refractive index detection