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Figure 7 | BMC Biotechnology

Figure 7

From: Active fungal GH115 α-glucuronidase produced in Arabidopsis thaliana affects only the UX1-reactive glucuronate decorations on native glucuronoxylans

Figure 7

Immunolabeling of the basal stem sections with UX1 and AX1 antibodies. The 0.5 μm sections of WT (A), transgenic plants line 4 (B) and line 5 (C), and the (Me)GlcA-deficient mutant, gux1gux2 (D). The sections were pretreated either with 0.05 M NaOH overnight before the labeling in order to reveal antibody binding to de-acetylated samples or with water for the same duration to analyze the antibody binding in the acetylated samples. Note that UX1-reactive sites in native samples were absent in ScAGU115 expressing lines. Bar = 50 μm.

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BMC Biotechnology

ISSN: 1472-6750

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