Figure 5
Transcript analysis of T2 transgenic A. thaliana after induction with 17-β-estradiol. (A) Agarose gel following RT-PCR with yoeB toxin and the control Actin primers from four different plants transformed with pMDC221_yoeBGFP and wild type control 24 hours after induction; (B) expression levels of the yoeB toxin in A. thaliana plants transformed with pMDC221_yoeBGFP from day 1 – day 7 after induction as determined by qRT-PCR. Each bar represents the mean and standard error for 3 biological replicates which were chosen randomly from 100 T2 transgenic plants from line 1.