Skip to main content

Table 2 Elastic penetration moduli of recombinant spidroin-based fusion protein layers

From: In planta production of ELPylated spidroin-based proteins results in non-cytotoxic biopolymers

Substance

Elastic penetration modulus E (GPa)

1xVSO1-100xELP

2.73 ± 0.02

2xVSO1-100xELP

3.85 ± 0.02

4xVSO1-100xELP

4.51 ± 0.03

LDPE Riblene®FL30

0.172 ± 0.001

  1. GPa: Gigapascal; ELP: elastin-like peptide.
  2. Values were obtained by AFM-based nanoindentation under the following conditions: temperature = 23°C, relative humidity = 35%, system sensitivity = 22.5 nm/V, spring constant = 59.3 N/m, tip radius = 112 nm, setpoint control = 500 nN, relative setpoint = 2500 nN, z-closed loop active, z-length = 1 μm, retract delay = 10 ms, grid size = 25 × 25 μm2, data point 35 × 35 per run with an orthogonal and lateral inter-sampling point distance of at least 715 nm. Low density polyethylene (LDPE) Riblene® FL30 foil was used as reference.
Back to article page

BMC Biotechnology

ISSN: 1472-6750

Contact us