Figure 3

Detection of TALEN-induced mutations and their sequences. (A) Mutations in Dll were detected by T7EI assay. Arrowheads indicate the cleavage products generated in T7EI assay of F₀ founders. (B) Sequence analysis of Dll mutant alleles in F₀ founders induced by TALENs. The wild type (WT) sequence is shown at the top with the TALEN-binding sites (highlighted in gray). The number of nucleotides deleted (-) and inserted (+) is indicated in parentheses. (C) Sequence analysis of Dll mutant alleles in F₂ embryos. Binding sites and insertions/deletions indicated as in (B).