Figure 5From: Evaluation of the impact of single nucleotide polymorphisms and primer mismatches on quantitative PCRAssay comparison for genotyping. The discrimination potential of Assay1 (A, B) and Assay2 (C, D) was evaluated with 10 ng of genomic DNA. Panels A and C represent the number of molecules predicted for known genotypes according to the priming probabilities presented in Table 3. The error bars in panels A and C are 99% confidence intervals associated with each value. Panels B and D are the results obtained from individual trees of unknown genotypes. The (+) and (-) above each bar indicate whether the observed number of molecules is within (+) or outside (-) of the 99% confidence interval from panels A and C, respectively. This criterion was used to discriminate between the presence or absence of an allele. Thus, comparison of panels A and C enables genotyping with Assay1: the numbers of molecules for Trees 14, 17, 20 are within the predicted intervals for a GT/GG heterozygote. Similarly, the numbers of molecules for Trees 2, 4, 6 are within the intervals predicted for a GT/GT homozygote. In contrast, the numbers of molecules predicted for all genotypes with Assay2 are too similar to one another to assign genotypes.Back to article page