Figure 3
From: Luciferase activity under direct ligand-dependent control of a muscarinic acetylcholine receptor
Functional integration of luciferase into the M 3 muscarinic acetylcholine receptor. A) COS-7 cells were transfected with luciferase construct, M3R-luci and construct #8 and luciferase activity was determined after incubation without and with the indicated ligands. The luminescences without ligands were 1,905,212 ± 172,463 AU (luciferase), 196,512 ± 4,942 AU (M3R-luci) and 100,473 ± 9,770 AU (#8). All data are given as means ± SEM of three independent experiments each performed in triplicate. B) Basal and CCh-induced IP formation was determined in COS-7 cells transfected with the wild-type M3R, M3R-luci and construct #8. All data are given as means ± SEM of three independent experiments each performed in triplicate.