Figure 7

Transgene expression of re-established hMSC-NT3-EGFP cultures from hMSC-NT3-EGFP cell transplanted spinal cords. (A) Flow cytometric assessment of EGFP expression (Y-axis) by an established cell culture at passage 1 from dissected hMSC-NT3-EGFP cell transplanted spinal cords showing the presence of both EGFP positive and EGFP negative cells. (B) Flow cytometric staining for human CD29 (X-axis) and human CD73 (= Y-axis) demonstrating that EGFP positive cells originate from hMSC-NT3-EGFP cell transplants while EGFP negative cells do not stain positive for human antibodies. Representative flow cytometric data were chosen from multiple re-established hMSC-NT3-EGFP cell cultures (n = 3).