Hepatic ERα mRNA levels after Ad-mediated transfer of shERα in vivo. Male C57Bl/6 mice (n = 5) were injected with 4 × 109 pfu Ad.Empty, Ad.shERα_1395 or Ad.shERα_tandem. Livers were harvested Four days after Ad. administration and subjected to taqman analysis. The cyclophillin gene was used as internal standard. Data represented as mean ± SD.