Table 1 PCR primers used in this study
From: The Flp double cross system a simple efficient procedure for cloning DNA fragments
# | Primer name | Primer sequence (5'-3') | Features | Amplifies |
|---|---|---|---|---|
1. | FRT 5' (Bam) LacZ | tcaggcgcgccgtcgaccatatg gaagttcctatac tttctaga gaataggaacttc g gaataggaacttc GGATCC caaaccgcctctccccgcgc | Buffer sequence FRT site BamHI site lac sequence 97 nt | 591 bp of lac promoter, operator and lacZ (including primers). 5' end begins at nt# 1081 of J01636 (GI146575) |
2. | FRT inverse 3' (RI) LacZ | catggcgcgccgtcgaccatatg gaagttcctatac tttctaga gaataggaacttc g gaataggaacttc GAATTC ccggaaaccaggcaaagcg | Buffer sequence FRT site EcoRI site lacZ sequence 96 nt | 591 bp of lac promoter, operator and lac Z (including primers). 5' end begins at nt# 1479 of J01636 (GI146575) |
3. | FRT 5' (Bam) KvDMR | tcaggcgcgccgtcgaccatatg gaagttcctatac tttctaga gaataggaacttc g gaataggaacttc GGATCC agtgggggcttcagaacatc | Buffer sequence FRT site BamHI site KvDMR sequence 97 nt | 1679 bp of intron 10 of human KCNQ1 gene(including primers). 5'end begins at nt #68364 of accession # U90095 |
4. | FRT inverse 3' (RI) KvDMR | catggcgcgccgtcgaccatatg gaagttcctatac tttctaga gaataggaacttc g gaataggaacttc GAATTC gattccagactccaatccca | Buffer sequence FRT site EcoRI site KvDMR sequence 97 nt | 1679 bp of intron 10 of human KCNQ1 gene (including primers). 5'end begins at nt #66879 of accession # U90095 |