Quantitative Real Time Polymerase Chain Reaction for measurement of human Interleukin – 5 receptor alpha spliced isoforms mRNA

Table 2 Accuracy of the standard curve for hIL-5Rα spliced forms. Coefficients of variation for C_T values and calculated quantities.

	C_T standard	C_T unknown	Qty standard	Qty unknown	C.V. (C_T)	C.V. (Qty)
Membrane-anchored hIL-5Rα	21.86	21.75	3.00 × 10⁴	3.02 × 10⁴	0.94	2.20
	24.96	24.92	3.00 × 10³	3.12 × 10³	0.35	4.08
	28.02	28.09	3.00 × 10²	3.37 × 10²	0.34	7.39
	31.88	31.92	3.00 × 10¹	2.79 × 10¹	0.60	9.19
Soluble hIL-5Rα	22.44	22.05	3.00 × 10⁴	4.49 × 10⁴	1.19	23.91
	24.71	24.65	3.00 × 10³	2.94 × 10³	0.59	2.02
	27.09	27.17	3.00 × 10²	2.80 × 10²	0.32	4.00
	31.60	31.15	3.00 × 10¹	2.61 × 10¹	0.83	9.29

Mean of C_T values and quantities (number of molecules) after amplification of the transcripts encoding for the soluble and membrane-anchored hIL-5Rα as standards and as unknowns. C.V(C_T): coefficient of variation in % for C_T values of standards and unknowns. C.V (Qty): coefficient of variation in % between input (Standards) and obtained quantities (unknowns).

ISSN: 1472-6750