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Table 2 Accuracy of the standard curve for hIL-5Rα spliced forms. Coefficients of variation for CT values and calculated quantities.

From: Quantitative Real Time Polymerase Chain Reaction for measurement of human Interleukin – 5 receptor alpha spliced isoforms mRNA

  CT standard CT unknown Qty standard Qty unknown C.V. (CT) C.V. (Qty)
Membrane-anchored hIL-5Rα 21.86 21.75 3.00 × 104 3.02 × 104 0.94 2.20
  24.96 24.92 3.00 × 103 3.12 × 103 0.35 4.08
  28.02 28.09 3.00 × 102 3.37 × 102 0.34 7.39
  31.88 31.92 3.00 × 101 2.79 × 101 0.60 9.19
Soluble hIL-5Rα 22.44 22.05 3.00 × 104 4.49 × 104 1.19 23.91
  24.71 24.65 3.00 × 103 2.94 × 103 0.59 2.02
  27.09 27.17 3.00 × 102 2.80 × 102 0.32 4.00
  31.60 31.15 3.00 × 101 2.61 × 101 0.83 9.29
  1. Mean of CT values and quantities (number of molecules) after amplification of the transcripts encoding for the soluble and membrane-anchored hIL-5Rα as standards and as unknowns. C.V(C T ): coefficient of variation in % for CT values of standards and unknowns. C.V (Qty): coefficient of variation in % between input (Standards) and obtained quantities (unknowns).