Figure 3

Validation of the position of the integrated plasmid, pMS98, in the S. coelicolor genome. Primers PB4 and PB4rev were designed to flank the SV1 attB site and were predicted to amplify a 867 bp fragment as shown in the agarose gel depicted on the left. Using pairs of primers PB4rev with PB3rev and PB3 with PB4, the attL and attR sites were amplified from S. coelicolor J1929:pMS98 genomic DNA. Fragments of the predicted sizes were obtained as depicted in the agarose gel (right).