Figure 2

Zymogram analysis of ADH isozymes from GX-UN120 during ethanol fermentation. Fermentation was performed in YPD containing 150 g/L glucose at 40°C. Cells were harvested from the broth at the lag (4 h), exponential (24 h) and stationary phases (72 h) and disrupted by grinding on ice. The cell extracts were separated on 7.5% polyacrylamide gel and the gels were stained for ADH activity with ethanol as the substrate.