Table 3 Strains and plasmids used in this study
Strains & plasmids | Properties | Source/reference |
|---|---|---|
E. coli | Â | Â |
JM109 | Cloning host | Our laboratory |
BL21(DE3) | F-, ompT, hsdS(rBB-mB-), gal, dcm (DE3) | Our laboratory |
C. glutamicum | Â | Â |
ATCC13032 | Wild-type | Our laboratory |
ATCC 15940 | L-Proline production | Our laboratory |
ATCC 21355 | L-Proline production | [27] |
ATCC 21157 | L-Proline production | [27] |
49-1 Plasmids | L-Proline production | Our laboratory |
pET-28a | His4-tag, T7 promoter, Kanr | Our laboratory |
pEC-XK99E | E. coli - C. glutamicum shuttle expression vector, Kanr | Our laboratory |
pET-28a-p4hP | pET-28a containing the p4h gene from P. stutzeri | This study |
pEC-XK99E-p4hP | pEC-XK99E containing the p4h gene from P. stutzeri | This study |
pET-28a-p4hB | pET-28a containing the p4h gene from B. bronchiseptica | This study |
pEC-XK99E-p4hB | pEC-XK99E containing the p4h gene from B. bronchiseptica | This study |
pET-28a- p4hD | pET-28a containing the p4h gene from Dactylosporangium sp. | This study |
pEC-XK99E-p4hD | pEC-XK99E containing the p4h gene from Dactylosporangium sp. | This study |