Figure 3

Schematic representation of different eYFP and luciferase expression constructs with various 5’ and 3’ UTRs. EMCV IRES, Encephalomyocarditis virus internal ribosomal entry site; BYDV 5’ UTR, Barley yellow dwarf virus 5’ untranslated region; BPH 5’ UTR, Baculovirus polyhedrin gene 5’ untranslated region; Ω, Tobacco mosaic virus 5’ leader sequence (omega); GAA_Ω, Tobacco mosaic virus 5’ leader sequence (omega) with GAA as first nucleotide triplet; GAA_E02, synthetic 5’ leader sequence; TMV, Tobacco mosaic virus; 5’ UTR*, repetition of a sequence complementary to the 18S ribosomal RNA; 3’ UTR*, trailer sequence with synthetic poly(A) signal; FFLuc, firefly luciferase; RRLuc, Renilla reniformis luciferase.