- Research article
- Open Access
A novel self-assembled oligopeptide amphiphile for biomimetic mineralization of enamel
- Quan-Li Li†1Email author,
- Tian-Yun Ning†2,
- Ying Cao1, 2,
- Wei-bo Zhang1,
- May Lei Mei2 and
- Chun Hung Chu2Email author
© Li et al.; licensee BioMed Central Ltd. 2014
- Received: 2 April 2013
- Accepted: 17 April 2014
- Published: 27 April 2014
Researchers are looking for biomimetic mineralization of ena/mel to manage dental erosion. This study evaluated biomimetic mineralization of demineralized enamel induced by a synthetic and self-assembled oligopeptide amphiphile (OPA).
The results showed that the OPA self-assembled into nano-fibres in the presence of calcium ions and in neutral acidity. The OPA was alternately immersed in calcium chloride and sodium hypophosphate solutions to evaluate its property of mineralization. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) showed nucleation and growth of amorphous calcium phosphate along the self-assembled OPA nano-fibres when it was repetitively exposed to solutions with calcium and phosphate ions. Energy dispersive spectrometry (EDS) confirmed that these nano-particles contained calcium and phosphate. Furthermore, electron diffraction pattern suggested that the nano-particles precipitated on OPA nano-fibres were comparable to amorphous calcium phosphate. Acid-etched human enamel slices were incubated at 37°C in metastable calcium phosphate solution with the OPA for biomimetic mineralization. SEM and X-ray diffraction indicated that the OPA induced the formation of hydroxyapatite crystals in organized bundles on etched enamel. TEM micrographs revealed there were 20–30 nm nano-amorphous calcium phosphate precipitates in the biomimetic mineralizing solution. The particles were found separately bound to the oligopeptide fibres. Biomimetic mineralization with or without the oligopeptide increased demineralized enamel microhardness.
A novel OPA was successfully fabricated, which fostered the biomimetic mineralization of demineralized enamel. It is one of the primary steps towards the design and construction of novel biomaterial for future clinical therapy of dental erosion.
Dental erosion is described as tooth surface loss produced by acids of non-bacterial origin and is now recognized as a major cause of tooth wear . The treatment particular in advanced cases is complicated and difficult and is a great challenge to many dentists. Regeneration by biomimetic mineralization is a non-invasive therapeutic technique that has received increasing attention in the past decades . A cell-free strategy of biomimetic mineralization has been proposed [2, 3]. Researchers tried to mimic the natural process for biomineralization and developed inorganic–organic hybrid materials with a hierarchical microstructure that simulates the natural mineralized tissues . This biomimetic mineralization method draws attention to the design of organic molecules as templates for controlling the nucleation and growth of mineral crystals and the design of the form of mineral-ion transport. It incorporates these two factors to form hierarchical hybrid materials under physiologic conditions [5, 6]. Various in situ methods of biomimetic mineralization with promising results to regenerate the hierarchical microstructure of the enamel have been reported. The methods utilized gelatine gel loaded with calcium and phosphate ions , hydrogen peroxide containing calcium phosphate paste , hydrothermal method with control release of calcium from Ca-EDTA , surfactant assembly hydroxyapatite , self-assembly of amphiphilic dendrons , electrolytical deposition taking place at 85°C , and amelogenin .
Matured enamel is composed of 95 wt% nano-rod-like hydroxyapatite. It is crucial to design the macromolecules as the template to induce the enamel microstructure regeneration under physiological conditions . During enamel formation, amelogenin, the major enamel protein constituting approximately 90% of all organic matrix material , spontaneously self-assembles into nano-spherical supermolecular structures. Amelogenin provides an effective template to induce enamel microstructure regeneration. However, it can be easily denatured and expensive to extract. Oligopeptides are less costly to prepare and more stable than natural protein. This biomaterial has been used as a molecular tool for the synthesis, growth, and assembly of nano-structured materials . Hartgerink et al. developed a peptide amphiphile that contains an alkyl tail and an ionic oligopeptide . The peptide amphiphile is able to self-assemble into nano-fibre networks. It can promote the mineralization of hydroxyapatite to form a composite material in which the crystallographic c-axes of hydroxyapatite are aligned with the long axes of the fibres. Kirkham et al. developed an oligomeric β-sheet-forming peptide that spontaneously undergoes hierarchical self-assembly into fibrillar scaffolds. It can induce hydroxyapatite nucleation de novo and facilitate remineralization of caries-like lesions .
Amelogenin spontaneously self-assembles into nano-spherical, supermolecular structures during enamel formation. Afterwards, it self-assembles into birefringent, microribbon structures . This supermolecular structure plays a vital role in controlling enamel hydroxyapatite crystal co-assembly . The molecular mechanism of amelogenin self-assembly involves the primary structure of the protein maintaining a bipolar nature, which leads to unique globular monomers with flexible, hydrophilic, C-terminal (-Thr-Lys-Arg-Glu-Glu-Val-Asp) “tails” on globular monomer surfaces . The hydrophilic C-terminal “tail” interacts with calcium ions and initializes hydroxyapatite nucleation . This study aimed to fabricate a self-assembly anionic oligopeptide amphiphile that contains the hydrophilic functional domain of amelogenin to initialize hydroxyapatite nucleation and promote biomimetic mineralization of demineralized enamel.
Oligopeptide amphiphile fabrication
The oligopeptide amphiphile (C18H35O-Thr-Lys-Arg-Glu-Glu-Val-Asp) fabricated in this study consists of “two parts” hydrophilic C-terminal amino residue of amelogenin (-Thr-Lys-Arg-Glu-Glu-Val-Asp) and a derivative of stearic acid (C18H35COOH) (Sigma-Aldrich, St. Louis, MO, USA). The amino residue of amelogenin was used as the hydrophilic functional group, and the stearic acid derivative was used as the hydrophobic tail. The oligopeptide amphiphile was synthesized using standard, Fmoc solid-phase, peptide synthesis (SPPS, Additional file 1: Figure S1), which proceeded step by step in a C-terminal to N-terminal fashion using automated synthesizers. The synthetic oligopeptide was purified by ammonium sulphate precipitation and reverse-phase, high-performance liquid chromatography (HPLC). It was characterized by high-performance liquid chromatography (HPLC) and mass spectrometry.
Self-assembly of the oligopeptide induced by calcium ions
Oligopeptide solutions at concentrations of 1, 2.5, 5, or 10 mg/mL were prepared by dissolving the oligopeptide into 0.1 M sodium hydroxide solution (Sigma-Aldrich, St. Louis, MO, USA). The alkalinity of the oligopeptide solution was then adjusted to neutral (pH = 7.0) by adding hydrochloric acid. A 100 μL aliquot of a 1 M calcium chloride (CaCl2) solution (Sigma-Aldrich, St. Louis, MO, USA) was added to 1 mL of the oligopeptide solutions at concentrations of 1, 2.5, 5.0, or 10.0 mg/mL. A suspension was formed and mixed thoroughly in a vial after each drop of the CaCl2 solution to promote diffusion of the metal ions. The self-assembled oligopeptide was characterized by transmission electron microscopy (TEM) and scanning electron microscopy (SEM) as the following. A suspension of 10 mg/mL self-assembled oligopeptide was ultrasonically diluted with ethanol. The diluted suspension was smeared onto a carbon-coated TEM grid and examined under TEM (JEOL-2010, JEOL Ltd. Co., Tokyo, Japan). For SEM examination (Sirion 200, FEI Co., Hillsboro, OR, USA), the oligopeptide-coated TEM grids were fixed with 2% glutaraldehyde in phosphate-buffered saline (PBS) (pH = 7.4) at 23°C for 2 h. It was subsequently dehydrated in ethanol and critical-point dried before gold sputtering.
Biomimetic mineralization property of the oligopeptide nano-fibres
Self-assembled oligopeptide was deposited onto a porous carbon-coated TEM grid and fixed with 2% glutaraldehyde in PBS (pH = 7.4) at 23°C for 2 h. Grids coated with the oligopeptide were first immersed in a 10 mM CaCl2 solution for 1 h at 23°C and then washed with deionized water. Afterwards, the samples were exposed to a 5 mM sodium hypophosphate (Na2HPO4) solution for 1 h at 23°C and washed with deionized water. The protocol of CaCl2 followed by Na2HPO4 immersion was repeated five times. The specimens were examined under TEM and SEM to study the biomimetic mineralization of the oligopeptide nano-fibres. Electron diffraction was used to study the crystal structure. Energy dispersive spectrometry (EDS) was used to evaluate the calcium and phosphorus content.
Biomimetic mineralization of enamel in metastable calcium phosphate solution
Sound extracted human molars were collected with ethics approval (IRB UW 10–210). Eight enamel slices of 1 mm in thickness were prepared perpendicular to the longitudinal axis of the tooth using a low-speed IsoMet diamond saw (Buehler Ltd., Lake Bluff, IL, USA). The slices were polished with 1200-grit silicon carbide paper under running water. The slices were checked under microscope (×10) for cracks before they were cleaned in ethoxylate detergent solution, acetone, ethanol, and deionized water. The enamel slices were demineralized by acid-etching with 37% phosphoric acid (H3PO4) for 60 s, rinsed with deionized water, and stored in a polyethylene tube at 4°C.
The metastable calcium phosphate solution was prepared as suggested by Fan . It contained 2.58 mM calcium (CaCl2 · 2H2O), 1.55 mM phosphates (KH2PO4), 1 mg/L sodium fluoride (NaF), and 180 mM sodium chloride (NaCl). It was buffered to a pH of 7.6 by 50 mM of trihydroxymethylaminomethane (Tris)-hydrochloric acid (Sigma-Aldrich, St. Louis, MO, USA). The biomimetic mineralizing solution was developed by adding oligopeptide. It was added to the buffered metastable calcium phosphate solution to achieve a concentration of 15 μg/mL. In order to reveal the particle size distribution of Ca2+-induced self-assembled peptide, the biomimetic mineralizing solution was characterized at 25°C by a nano-particle size analyser (Zetasizer Nano S90, Malvern Instruments Ltd, UK). Particle size distribution was studied using multimodal algorithms based on the size distribution of the scattering light intensity. The stability of the biomimetic mineralizing solution and the metastable calcium phosphate solution were assessed by examination for precipitation after 30 days. The precipitate formed, if any, was studied using TEM.
Two tooth slices were placed into a polyethylene tube containing 15 mL biomimetic mineralizing solution and incubated at 37°C for 1 day. Another 2 slices were incubated for 20 days. The biomimetic mineralizing solution was refreshed every 2 days. The tooth slices after incubation were washed with deionized water and air-dried before examination under SEM and X-ray diffraction (XRD) (X’Pert Pro, Philips, Almelo, The Netherlands). A microhardness tester (Type M, Shimadzu Corp., Kyoto, Japan) was used to measure the Vicker’s hardness on the enamel of the tooth slices. The enamel surface was subjected to a load of 100 × 10-3N for 10 s at each test point for microhardness assessment. The above experiments were repeated on 4 tooth slices using the biomimetic mineralizing solution without the oligopeptide as a control.
The collected data was entered into an Excel file and checked to minimize data entry errors. The data was then analysed with statistical software SPSS 17.0 for Windows (SPSS Inc. Chicago, Illinois, USA). GLM univariate analysis will be used to check normality and variance of the samples. One-way analysis of variance and Tukey’s test were used to compare the microhardness of the enamel surface i) with or without H3PO4 etching enamel and ii) after incubation in biomimetic mineralizing solution with or without the oligopeptide. The level of significance was 5%.
Oligopeptide amphiphile fabrication
Self-assembly of the oligopeptide induced by calcium ions
Biomimetic mineralization property of the oligopeptide nano-fibres
Biomimetic mineralization of enamel in metastable calcium phosphate solution containing the peptide
Figure 9a showed XRD spectra of enamel samples. The diffraction peaks (002), (211), (112), and (300) planes corresponded well to the peaks for hydroxyapatite. The ratios of diffraction intensity of the c-axis (002) reflection to the diffraction intensity of the (211) or a-axis (300) reflections were considerably enhanced in all the calcium phosphate-coated samples compared to the etched tooth surface. The result suggested that the precipitated hydroxyapatite was oriented along its c-axis. The size of the precipitate crystal along the c-axis (length) and a-axis (width) was calculated using the Debye-Scherrer formula, D = 0.89λ/(β1/2 * cosθ) (D is the crystallite size; λ is the wavelength of the X-rays; and β1/2 is the diffractometry (XRD) pattern broadening). The length of the crystal was shorter in the presence of the oligopeptide than that formed without the oligopeptide. The width of the crystal was affected to a lesser extent. The results match the observations from the SEM (Figure 10).
The oligopeptide used in this study is fabricated by SPPS, one the most common techniques for peptide synthesis, which proceeded step by step in a C-terminal to N-terminal fashion and ended with the alkylation of the NH2 terminus of the peptide . HPLC and mass spectrometry were used to characterize and evaluate the purity of the oligopeptide . This study demonstrated that it can be synthesized and purified to a high degree of purity although it is a relatively long molecule. The synthetic oligopeptide is amphiphilic because it has a hydrophilic anionic oligopeptide with a hydrophobic tail. The stearic acid derivative has a long carbon alkyl chain which was used as the hydrophobic tail. It was reported that the alkyl tail attached to the NH2 or COOH termini of peptide could influence their aggregation and secondary structure in water in both synthetic and natural systems . The hydrophobic alkyl tails would pack together in the centre and leave the peptide segments exposed to the aqueous environment, resulting in peptide amphiphile self-assembly. The amino acid sequence of the peptide derived from the hydrophilic C-terminal amino residue of amelogenin and acts as a functional domain to induce mineralization. The oligopeptide can be dissolved in an alkaline solution but is insoluble in neutral or acidic solutions, which may contribute to its hydrophilic anionic peptide sequence. The results suggested that calcium ions induced the oligopeptide to self-assemble. It is reported that it is an essential step of supermolecular assembly for the oligopeptide to act function [18, 19]. We considered that the property of calcium ions’ induced self-assembly may facilitate the mineralization to capture and trap calcium ions within the mineralized areas. Although the results cannot confirm that the self-assembly oligopeptide is able to capture calcium and phosphate ions from the solutions and to initiate the nucleation, this study demonstrated the peptide can induce ACP to form along the self-assembly fibres when it is alternately immersed in a calcium ions solution and a phosphate ions solution. Quantitative assessment of the calcium and phosphate content of ACP such as atomic absorption spectroscopy is essential, but the quantity of mineralized peptide fibres was too little for assessment.
The biomineralization process is an organic matrix-mediated mineralization other than a simple thermodynamic and kinetic process of inorganic mineral ions crystallization under aqueous solution condition . A key aspect of this organic matrix-mediated deposition concerns the role of molecular interactions in controlling oriented nucleation at the matrix-mineral interface. In this study, the flocculent precipitates containing ACP and apatite were observed in the metastable calcium phosphate solution without oligopeptide addition after 30 days. This is probably because ACP spheres were partly transformed into apatite crystallite clusters. However, the calcification solution with oligopeptide was relatively clear with few precipitates of 20–30 nm nano-ACP particles over 30 days. No apatite crystal was observed. This suggests that the nano-ACP precursor is obtained by adding the oligopeptide to metastable calcium phosphate solutions with fluoride. The negatively charged peptide binds to ACP nano-precursors and sequesters the ions. There is inhibition of crystal nucleation and induction of liquid-liquid-phase separation in the aqueous crystallizing solution [25, 26]. The sequestered ACP presents as droplets that appear transparent to the naked eye because they are smaller than the wavelength of light. Because of the sequestering mechanism, the oligopeptide-ACP complex can prevent ACP nano-precursors from aggregation and precipitation and make them small. Therefore, the nano-ACP precursor is highly active and can be transported to the mineralizing zone to induce mineralization .
More and more evidence supports the notion that biomineralization is a non-classical crystallization pathway and a bottom-up approach . In such cases, crystallization often proceeds by a sequential process involving structural and compositional modifications of amorphous precursors and crystalline intermediates. Crystallization often involves an initial amorphous phase (such as ACP) that may be non-stoichiometric, hydrated, and susceptible to rapid phase transformation . According to this concept, a biomimetic mineralization method has been successfully utilized in the duplication of a hard tissue structure . Acidic macromolecules are added to a metastable calcium phosphate solution to stabilize the amorphous mineral phases and to reduce these amorphous phases to the nanoscale . Crystal growth is then controlled by the macromolecules that undergo self-assembly. In this study, we examined the initial nucleation and growth (24 h) of the precipitated crystals and the speed of HA crystal growth (20 days) induced by the oligopeptide. It is more desirable to study the samples in multiple time point. It is our primary study and communication report, and more time point should be studied to explain the HA crystal growth in the following study.
In this study, the microhardness of the samples with hydroxyapatite precipitates in the presence of oligopeptide was similar to those without oligopeptide. Homogeneous hydroxyapatite crystals were oriented in parallel and packed together to form a dense, uniform layer. As the crystals on the surface were newly mineralized, they did not reach the mature stage of the crystal that formed underneath. This agrees with the findings reported by Busch  that there was no significant difference in hardness between the remineralization groups regardless of oligopeptide use.
Primary and secondary biomimetic growth of calcium phosphate can be distinguished on polymer surfaces. It is however not easy to distinguish the growth with the SEMs provided in this study. Mature enamel hydroxyapatite crystals bundled in parallel to form a special microstructure of repeating units of prisms. It is plausible that the structure of the precipitate was similar to the microstructure of the enamel. It is better to evaluate the remineralization with a quantitative method. However, the precipitate and the enamel had similar composite and structure; even the atomic absorption spectroscopy could not show the difference.
This study did not assess the stability or incorporation of the hydroxyapatite precipitates onto the enamel surface. In addition, the thickness of the crystal layer precipitated in the presence of the oligopeptide was less than that formed without oligopeptide. Therefore, the presence of the oligopeptide reduced the precipitation rate of hydroxyapatite crystal. It is noteworthy that the precipitated crystals with oligopeptide were more densely packed than those precipitated without oligopeptide. This is probably due to the different rate of crystal precipitation. The results showed that the morphology and the growth of the precipitated layer (remineralized layer) was different between the experimental samples (with the oligopeptide) and the control samples (without oligopeptide). Compared to the control samples, the advantage of using the oligopeptide is that the crystals are more densely packed. The disadvantage was that the deposition rate is slow in the presence of oligopeptide. However, the slow speed of crystal growth contributes to crystals packing densely. The metastable calcium phosphate solution with oligopeptide is more stable than the metastable calcium phosphate solution without oligopeptide. This advantage will facilitate its potential clinic use.
Bertassoni and his co-workers proposed that a more appropriate endpoint to evaluate the effectiveness of remineralization should be associated with the recovery of the mechanical properties of the hydrated dental tissue, which is presumed to correlate well with its overall functionality . Hardness testing is an indirect method of tracking changes in the mineral content of dentin . Biomechanical properties of dental tissues in general have been mostly evaluated by microhardness testing methods . A disadvantage in microhardness assessment is the lack of analysis of reading error . Although reading errors may have occurred in this study, the findings of microhardness measurement are consistent with the SEM and TEM observations. In addition, any reading error in this investigation should be small because the rater is experienced in microhardness testing and became fully acquainted with the procedure.
A novel self-assembly oligopeptide amphiphile was fabricated with high purity. The oligopeptide self-assembles into nano-fibres in the presence of calcium ions and at neutral acidity. The self-assembled oligopeptide allows precipitation of ACP along its nano-fibres when it was alternately immersed in a calcium ions solution and a phosphate ions solution. The metastable calcium phosphate solution with the oligopeptide can induce formation of a relatively stable nano-ACP complex precursor. The nano-ACP complex precursor can induce nano-rod hydroxyapatite growth which densely packed and bundled together to form enamel-like tissue on demineralized enamel and the precipitation of these crystals increases enamel hardness. This synthetic novel oligopeptide can be a potential molecular tool for biomimetic mineralization on the enamel surface. It is one of the primary steps towards the design and construction of novel biomaterial for future clinical care of dental erosion.
This study was supported by NSFC (No.30973352) and NSFC/RGC Joint Research Scheme (N_HKU 776/10 and No.81061160511), and Youth Foundation of Anhui Provincial Natural Science Foundation (No.1208085QH144).
- Chu CH, Pang KK, Lo EC: Dietary behavior and knowledge of dental erosion among Chinese adults. BMC Oral Health. 2010, 10: 13-10.1186/1472-6831-10-13.View ArticleGoogle Scholar
- Featherstone JD: Remineralization, the natural caries repair process-the need for new approaches. Adv Dent Res. 2009, 21: 4-7.Google Scholar
- Cochrane NJ, Cai F, Huq NL, Burrow MF, Reynolds EC: New approaches to enhanced remineralization of tooth enamel. J Dent Res. 2010, 89: 1187-1197. 10.1177/0022034510376046.View ArticleGoogle Scholar
- Yamane S, Sugawara A, Watanabe A, Akiyoshi K: Hybrid nanoapatite by polysaccharide nanogel-templated mineralization. J Bioact Compat Poly. 2009, 24: 151-168. 10.1177/0883911508101554.View ArticleGoogle Scholar
- Colfen H, Mann S: Higher-order organization by mesoscale self-assembly and transformation of hybrid nanostructures. Angew Chem. 2003, 42: 2350-2365. 10.1002/anie.200200562.View ArticleGoogle Scholar
- Niederberger M, Colfen H: Oriented attachment and mesocrystals: non-classical crystallization mechanisms based on nanoparticle assembly. Phys Chem Chem Phys. 2006, 8: 3271-3287. 10.1039/b604589h.View ArticleGoogle Scholar
- Busch S: Regeneration of human tooth enamel. Angew Chem. 2004, 43: 1428-1431. 10.1002/anie.200352183.View ArticleGoogle Scholar
- Yamagishi K, Onuma K, Suzuki T, Okada F, Tagami J, Otsuki M, Senawangse P: Materials chemistry: a synthetic enamel for rapid tooth repair. Nature. 2005, 433: 819-10.1038/433819a.View ArticleGoogle Scholar
- Chen H, Tang Z, Liu J, Sun K, Chang SR, Peters MC, Mansfield JF, Czajka-Jakubowska A, Clarkson BH: Acellular synthesis of a human enamel-like microstructure. Adv Mater. 2006, 18: 1846-1851. 10.1002/adma.200502401.View ArticleGoogle Scholar
- Fowler CE, Li M, Mann S, Margolis HC: Influence of surfactant assembly on the formation of calcium phosphate materials-A model for dental enamel formation. J Mater Chem. 2005, 15: 3317-3325. 10.1039/b503312h.View ArticleGoogle Scholar
- Yang S, He H, Wang L, Jia X, Feng H: Oriented crystallization of hydroxyapatite by the biomimetic amelogenin nanospheres from self-assemblies of amphiphilic dendrons. Chem Commun (Camb). 2011, 47: 10100-10102. 10.1039/c1cc13661e.View ArticleGoogle Scholar
- Ye W, Wang XX: Ribbon-like and rod-like hydroxyapatite crystals deposited on titanium surface with electrochemical method. Mater Lett. 2007, 61: 4062-4065. 10.1016/j.matlet.2007.01.040.View ArticleGoogle Scholar
- Fan Y, Sun Z, Wang R, Abbott C, Moradian-Oldak J: Enamel inspired nanocomposite fabrication through amelogenin supramolecular assembly. Biomaterials. 2007, 28: 3034-3042. 10.1016/j.biomaterials.2007.02.016.View ArticleGoogle Scholar
- Luo J, Ning T, Cao Y, Zhu X, Xu X, Tang X, Chu CH, Li L: Biomimic enamel remineralization by hybridization calcium- and phosphate-loaded liposomes with amelogenin-inspired peptide. Key Eng Mater. 2012, 512–515: 1727-1730.View ArticleGoogle Scholar
- Zhou Y-Z, Cao Y, Liu W, Chu CH, Li Q-L: Polydopamine-induced tooth remineralization. ACS Appl Mater Inter. 2012, 4: 6901-6910. 10.1021/am302041b.View ArticleGoogle Scholar
- Semino CE: Self-assembling peptides: from bio-inspired materials to bone regeneration. J Dent Res. 2008, 87: 606-616. 10.1177/154405910808700710.View ArticleGoogle Scholar
- Petka WA, Harden JL, McGrath KP, Wirtz D, Tirrell DA: Reversible hydrogels from self-assembling artificial proteins. Science. 1998, 281: 389-392.View ArticleGoogle Scholar
- Hartgerink JD, Beniash E, Stupp SI: Self-assembly and mineralization of peptide-amphiphile nanofibers. Science. 2001, 294: 1684-1688. 10.1126/science.1063187.View ArticleGoogle Scholar
- Kirkham J, Firth A, Vernals D, Boden N, Robinson C, Shore RC, Brookes SJ, Aggeli A: Self-assembling peptide scaffolds promote enamel remineralization. J Dent Res. 2007, 86: 426-430. 10.1177/154405910708600507.View ArticleGoogle Scholar
- Du C, Falini G, Fermani S, Abbott C, Moradian-Oldak J: Supramolecular assembly of amelogenin nanospheres into birefringent microribbons. Science. 2005, 307: 1450-1454. 10.1126/science.1105675.View ArticleGoogle Scholar
- Uskokovic V, Li W, Habelitz S: Amelogenin as a promoter of nucleation and crystal growth of apatite. J Cryst Growth. 2011, 316: 106-117. 10.1016/j.jcrysgro.2010.12.005.View ArticleGoogle Scholar
- Fan Y, Sun Z, Moradian-Oldak J: Controlled remineralization of enamel in the presence of amelogenin and fluoride. Biomaterials. 2009, 30: 478-483. 10.1016/j.biomaterials.2008.10.019.View ArticleGoogle Scholar
- Hosseinkhani H, Hosseinkhani M, Kobayashi H: Design of tissue-engineered nanoscaffold through self-assembly of peptide amphiphile. J Bioact Compat Poly. 2006, 21: 20-Google Scholar
- Fan Y, Wen TZ, Liao S, Lallier T, Hagan JL, Twomley JT, Zhang J-F, Sun Z, Xu X: Novel amelogenin-releasing hydrogel for remineralization of enamel artificial caries. J Bioact Compat Poly. 2012, 27: 14-Google Scholar
- Ning T-Y, Xu X-H, Zhu L-F, Zhu X-P, Chu CH, Liu L-K, Li Q-L: Biomimetic mineralization of dentin induced by agarose gel loaded with calcium phosphate. J Biomed Mater Res Part B. 2012, 100: 138-144.View ArticleGoogle Scholar
- Olszta MJ, Cheng X, Jee SS, Kumar R, Kim Y, Kaufman MJ, Douglas EP, Gower LB: Bone structure and formation: a new perspective. Mat Sci Eng R. 2007, 58: 77-116. 10.1016/j.mser.2007.05.001.View ArticleGoogle Scholar
- Cao Y, Mei ML, Xu JG, Lo EC, Li QL, Chu CH: Biomimetic mineralisation of phosphorylated dentine by CPP-ACP. J Dent. 2013, 41: 818-825. 10.1016/j.jdent.2013.06.008.View ArticleGoogle Scholar
- Gower LB: Biomimetic model systems for investigating the amorphous precursor pathway and its role in biomineralization. Chem Rev. 2008, 108: 4551-4627. 10.1021/cr800443h.View ArticleGoogle Scholar
- Jee SS, Thula TT, Gower LB: Development of bone-like composites via the polymer-induced liquid-precursor (PILP) process. Part 1: influence of polymer molecular weight. Acta Biomater. 2010, 6: 3676-3686. 10.1016/j.actbio.2010.03.036.View ArticleGoogle Scholar
- Bertassoni LE, Habelitz S, Kinney JH, Marshall SJ, Jr Marshall GW: Biomechanical perspective on the remineralization of dentin. Caries Res. 2009, 43: 70-77. 10.1159/000201593.View ArticleGoogle Scholar
- Chu CH, Lo EC: Microhardness of dentine in primary teeth after topical fluoride applications. J Dent. 2008, 36: 387-391. 10.1016/j.jdent.2008.02.013.View ArticleGoogle Scholar
- Chu CH, Lam A, Lo EC: Dentin hypersensitivity and its management. Gen Dent. 2011, 59: 115-122.Google Scholar
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