- Research article
- Open Access
Biocatalytic potential of vanillin aminotransferase from Capsicum chinense
© Weber et al.; licensee BioMed Central Ltd. 2014
- Received: 9 August 2013
- Accepted: 3 April 2014
- Published: 9 April 2014
The conversion of vanillin to vanillylamine is a key step in the biosynthetic route towards capsaicinoids in pungent cultivars of Capsicum sp. The reaction has previously been annotated to be catalysed by PAMT (putative aminotransferase; [GenBank: AAC78480.1, Swiss-Prot: O82521]), however, the enzyme has previously not been biochemically characterised in vitro.
The biochemical activity of the transaminase was confirmed by direct measurement of the reaction with purified recombinant enzyme. The enzyme accepted pyruvate, and oxaloacetate but not 2-oxoglutarate as co-substrate, which is in accordance with other characterised transaminases from the plant kingdom. The enzyme was also able to convert (S)-1-phenylethylamine into acetophenone with high stereo-selectivity. Additionally, it was shown to be active at a broad pH range.
We suggest PAMT to be renamed to VAMT (vanillin aminotransferase, abbreviation used in this study) as formation of vanillin from vanillylamine could be demonstrated. Furthermore, due to high stereoselectivity and activity at physiological pH, VAMT is a suitable candidate for biocatalytic transamination in a recombinant whole-cell system.
- Putative aminotransferase
- Whole-cell biocatalysis
Chili pepper fruit (Capsicum sp.) gives rise to a strong pungent sensation when eaten, due to the presence of capsaicinoids, with capsaicin (trans-8-methyl-N-vanillyl-6-nonenamide) as the most known example . Capsaicinoids have been considered as anti-obesity, antimicrobial and antineoplastic agents [1, 2], and for the prevention of hypertension . Additionally, capsaicin is a very potent agonist of the pain receptor TRPV1 [4, 5] and is therefore used as a therapeutic drug in the treatment of peripheral neuropathy. Since the discovery of capsaicinoids there has been substantial amount of research dedicated to the understanding of biosynthetic pathways leading to capsaicinoids in different cultivars of Capsicum sp. Although the biochemical pathways leading to capsaicin are described, there is still a knowledge-gap with regards to the identity of some of the involved enzymes (cf. review by Aza-González et al. ).
In this study, the gene encoding PAMT was functionally expressed in Escherichia coli and the polyhistidine-tagged enzyme was purified to homogeneity using affinity chromatography and subsequently characterised. It is confirmed that PAMT converts vanillylamine to vanillin, and should therefore from now on be called "vanillin aminotransferase (VAMT)". The enzyme can also be used for the kinetic resolution of racemic 1-phenylethylamine to (R)-1-phenylethylamine and acetophenone, and thus carries the potential to be applied for biocatalytic transamination for the preparation of chiral amines.
Cloning, expression and purification of VAMT
Strains and plasmids used in this study
Strains and plasmids
pUC 57 VAMT
Gene for VAMT
GenScript, NJ, USA
Plasmid with T7 promotor and terminator, Amp resistance gene
Invitrogen, CA, USA
VAMT under T7 promoter, with T7 terminator, Amp resistance gene
E. coli DH5α
Life Technologies, MD, USA
E. coli BL21(DE3) pLys
Invitrogen, CA, USA
E. coli TMB2100
E. coli BL21, containing pNW1
Functional validation of vanillin aminotransferase activity
Most known transaminases of both bacterial and plant origin accept L-alanine as amine donor [10–15]. The purified enzyme was therefore first evaluated for the conversion of vanillin to vanillylamine with a high excess of alanine as amine donor. It was, however, not possible to detect any formation of vanillylamine. Conversion was neither observed with the following amine donors: D-alanine, GABA, glutamine and ornithine. However it cannot be excluded that some vanillylamine (below the detection limit of our HPLC analysis) was formed since transamination of a small amount of vanillin was previously observed in crude cell extract from pungent pepper Capsicum annuum and with GABA as amine donor . However, the Gibbs free energy of the reaction, ΔG0’, as calculated by using the group contribution method , was ΔG0’ = +2.55 kcal/mol when using L-alanine and other amino acids as amine donor. The reaction was consequently thermodynamically favouring the reverse reaction and a high conversion of vanillin was, even with an excess addition of the amine donor, not expectable. It has previously been shown for other transaminases that a (co-) product removal system was required to reach conversion of aromatic ketones to amines [12, 17, 18]. Therefore, the enzyme was instead evaluated for the conversion of vanillylamine to vanillin using pyruvate as amine acceptor. In this case, the formation of vanillin could be detected shortly after the start of the reaction (the specific activity of the enzyme was approximately 5 U/mg protein), which indicates that VAMT indeed is responsible for vanillylamine formation in C. chinense. Whether this activity can be found in orthologous plant transaminases and is a general trait, or if it is a specific natural function of VAMT that has been evolved over time, is unknown. BlastP search within the NCBI database (http://blast.ncbi.nlm.nih.gov/Blast.cgi) with the amino acid sequence of VAMT identified γ-aminobutyrate (GABA) transaminases, catalysing the conversion of GABA to succinate semialdehyde as the most similar enzymes. The amino acid sequence of VAMT notably shared 76-83% identity to known plant GABA transaminases originating from Arabidopsis thaliana , apple  and tomato . None of these enzymes have been investigated for the ability to convert vanillin or vanillylamine. In fact, transamination of vanillin has, to the best of our knowledge, only been reported for the ω-transaminase from Chromobacterium violaceum DSM 30191 (CV-ωTA) ; however, transamination of numerous aryl substituted aldehydes and ketones have previously been described for ω-transaminases originating from various soil-living bacteria, for example ω-TAs from Aspergillus terreus, Hyphomonas neptunium, and Arthrobacter ATA-117 [14, 19]. It is likely that there are several other transaminases unrelated to VAMT that are able to convert vanillin to vanillylamine.
Potential for biocatalytic transaminations
To evaluate whether VAMT has any potential for biocatalytic transamination for the preparation of chiral amines, kinetic resolution of (rac)-1-phenylethylamine (1-PEA) was examined and which enantiomeric selectivity the enzyme possesses. The kinetic resolution of (rac)-1-PEA using pyruvate as amine acceptor was initiated by addition of the enzyme and was allowed to continue for 24 hours. The products were extracted with heptane and analysed using chiral HPLC. (S)-1-PEA was fully converted into acetophenone, while (R)-1-PEA remained, leading to an ee > 99% at 50% conversion of (rac)-1-PEA. Alike VAMT, most known transaminases that have been used for the generation of chiral amines are S-selective , with a few exceptions of R-selective enzymes [20, 21].
For the first time, the catalytic activity of vanillin transaminase from chili pepper plant Capsicum chinense was demonstrated with the purified enzyme in vitro. The enzyme displayed high transaminase activity towards vanillylamine and (S)-1-phenylethylamine with pyruvate or oxaloacetate as amine acceptor, and the enzyme was active at a broad pH range. Thus, VAMT can be used for the preparation of chiral amines, and represents an interesting candidate to be used in whole-cell biocatalysis with recombinant microorganisms.
Strains and cultivation conditions
E. coli strains and plasmids used in the study are listed in Table 1. E. coli strain DH5α was used for subcloning and transformants were selected on lysogeny broth (LB) media supplemented with 100 mg/l ampicillin (IBI Shelton Scientific, CT, USA). Strains were kept in 20% v/v glycerol stocks at -80°C and grown for 1 day on LB plates at 37°C before use. A single colony was picked and pre-cultured overnight in LB media and used for subsequent inoculation.
Standard molecular biology techniques were used for all cloning procedures . Plasmid DNA was prepared with the GeneJET™ Plasmid Miniprep Kit (Fermentas UAB, Vilnius, Lithuania). Agarose gel DNA extraction was made with QIAquick® Gel Extraction Kit (Qiagen GmbH, Hilden, Germany). Sequencing was performed by MWG-Biotech AG (Ebersberg, Germany). Restriction endonucleases and T4 DNA Ligase from Fermentas were used for DNA manipulation. Transformation of E. coli was performed with the calcium chloride  method.
The VAMT gene from C. chinense [GenBank: AAC78480.1, Swiss-Prot: O82521] was constructed synthetically (GenScript, NJ, USA), resulting in plasmid pUC57 VAMT (Table 1). The plasmid was digested with restriction enzymes BamHI and PstI, and the resulting fragment was inserted into pRSETB (Invitrogen, CA, USA). The resulting plasmid pNW1 was digested with BamHI, treated with Klenow fragment from Fermentas and re-ligated with T4 DNA Ligase from Fermentas to move the gene in frame. Correct orientations and sequences of the inserts were verified by restriction analysis and sequencing. pNW1 was transformed into E. coli BL21(DE3) pLys which gave strain TMB2100.
A single microbial colony of TMB2100 from a LB plate  containing 100 mg/l ampicillin was inoculated in 50 ml LB medium containing 100 mg/l ampicillin. Cells were grown overnight (at least 16 hr) in an incubator at 37°C with shaking at 180 rpm. The preculture was used to inoculate 250 ml LB medium containing 100 mg/l ampicillin in a 1 l shake flask with an optical density (OD620) of 0.1 and growth was performed at 37°C and 180 rpm. Expression of the transaminase gene VAMT was induced by addition of isopropyl-β-D-thiogalactoside (IPTG) to a final concentration of 1 mM when the OD620 of the culture reached 0.6 to 0.8 and incubation was then continued for 24 hours at 15°C and 180 rpm.
Purification of the transaminase
Cells of recombinant E. coli strain TMB2100 were harvested by centrifugation (3200 × g, 10 min) and washed once with 25 mL water. The cell pellet was resuspended in equilibration buffer, according to the manufacturer’s instructions, and subjected to sonication in a sonicator (Bandelin electronics, Berlin, Germany). Cell debris was removed by centrifugation (3200 × g, 10 min). The clear lysate supernatant was collected and passed through a nickel column (HisPur™ Ni-NTA Purification Kit, Thermo Scientific, Rockford, IL, USA), according to the description of the manufacturer. The purified transaminase was collected after elution with buffer, according to the manufacturer’s instructions. Protein concentration was determined using the Bradford method with bovine serum albumin (BSA) as a standard . Purity of the protein in the eluent was estimated with SDS-PAGE (Figure 2). The identity of the protein was confirmed by mass spectrometry at the SWEGENE proteomics platform (Lund, Sweden). The concentration of the purified transaminase was approximately 0.5 mg/ml.
Enzyme activity assay
Purified VAMT (0.5 ml) was mixed with 4 mM vanillylamine, (rac)-1-phenylethylamine (1-PEA) or vanillin, PLP (0.1 mM), and one of the following co-substrates (40 mM for amine acceptors, 100 mM for amine donors): pyruvate, oxaloacetate, 2-oxoglutarate, succinate, p-hydroxybenzaldehyde, acetone, L-alanine, D-alanine, GABA, glutamine or ornithine. The reaction was performed in 1 ml sodium phosphate buffer (100 mM, pH 6-7), Tris-HCl buffer (100 mM, pH 7-9) or CAPS buffer (100 mM, pH 10-12) in a glass vial. The reaction was started by the addition of the substrate and it was quenched by the addition of 0.1 ml 1 M HCl. Samples were kept at -20°C until analysis. Thawing of samples (0.5 ml) was done at room temperature and 1-PEA and acetophenone (ACP) were extracted with 0.6 ml heptane, after addition of 30 μl NaOH (10 M), and analyzed by HPLC. The specific activity of the enzyme was calculated based on the rate of vanillylamine conversion (1 U = conversion of 1 μmol vanillylamine per minute). Experiments were done at least in duplicates. The enzyme has a selectivity factor, E > 100 which was calculated according to where c = conversion, ee = enantiomeric excess).
Quantitative analysis of vanillin and vanillylamine were performed with a 1100 Series HPLC instrument (Agilent Technologies, Heilbronn, Germany) equipped with a quaternary pump, autosampler, diode array detector (DAD), fluorescence detector and a vacuum degasser. Chromatographic separation took place in a Zorbax SB-C18 (2.1 mm × 150 mm × 3.5 μm) column (Agilent, USA). A gradient elution method was optimized using two mobile phases, A (0.5% formic acid in water) and B (0.5% formic acid in methanol). The elution gradient started with an initial composition of 4% B and increased to 96% B within 5 min. The late composition was kept isocratic for 7 min, followed by returning back to initial composition in one minute. The method was ended with a 12 min equilibration step at the same composition resulting in a 25 min total runtime. Using the autosampler syringe, 5 μl sample was injected to the column. The mobile phase flow rate kept constant at 0.2 ml/min while monitoring with DAD at 280 nm wavelength. Processing the HPLC data was done using Agilent Chemstation revision B.01.03 software.
(R)- and (S)-1-phenylethylamine and acetophenone were determined using a Waters HPLC system (Binary HPLC pump 1525, UV/Vis detector 2489, Autosampler 2707) equipped with a Daicel ChiralCel OD-H column (4.6 × 25 mm, 5 μm). The mobile phase was 85:15 heptane/isopropanol with 0.1% butylamine and the flow rate was kept constant at 1 ml/min while monitoring at 210 nm wavelength. Using the autosampler syringe, 5 μl sample was injected to the column. All sample analyses were performed at room temperature. Retention times: (R)-1-phenyethylamine: 5.6 min; (S)-1-phenylethylamine: 6.8 min; acetophenone: 4.6 min.
The Swedish Research Council FORMAS and The Royal Swedish Academy of Agriculture and Forestry are acknowledged for financial support. This research was also supported by BIOFLAVOUR, COST Action FA0907 (http://www.bioflavour.insa-toulouse.fr).
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