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Table 3 Effects of cations and chemical reagents on purified Tth Man5 β-mannosidase activity

From: High-level expression of a novel thermostable and mannose-tolerant β-mannosidase from Thermotoga thermarum DSM 5069 in Escherichia coli

Cationsa

Residual activity (%)

Control

100

Mg2+

114.85±5.50

Zn2+

25.63±2.20

Mn2+

129.61±2.88

Ba2+

107.96±3.84

Ca2+

103.01±2.33

Al3+

109.61±3.50

Cu2+

28.93±1.22

Co2+

191.94±7.27

Ni2+

81.07±0.69

Chemical reagentsb

 

EDTA

119.32±1.24

Tween 60

100.19±3.76

Tris

58.34±0.96

SDS

26.02±0.51

  1. aFinal concentration, the former value in the table was determined at 1 mM. bFinal concentration, the values in the table were determined at 1 mM, 0.05%, 0.05% and 0.1% for EDTA, Tween 60, Tris and SDS, respectively. Values shown were the means of triplicate experiments.
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BMC Biotechnology

ISSN: 1472-6750

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