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Figure 2 | BMC Biotechnology

Figure 2

From: High-level expression of a novel thermostable and mannose-tolerant β-mannosidase from Thermotoga thermarum DSM 5069 in Escherichia coli

Figure 2

SDS-PAGE analysis of recombinant Tth Man5 β-mannosidase in E. coli BL21 (DE3). Lane M: protein marker, lane 1: cell-free extract of E. coli BL21 (DE3) harboring pET-20b plasmids, lane 2: the purified Tth Man5 β-mannosidase eluted with 0.2 M 1 mL imidazole buffer, lane 3,4: the purified Tth Man5 β-mannosidase eluted with 0.4 M 1 mL imidazole buffer (lane 3: first tube collection, lane 4: second tube collection).

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BMC Biotechnology

ISSN: 1472-6750

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