Skip to main content

Table 1 Targetron insertion sites and scores

From: Rapid targeted gene disruption in Bacillus anthracis

Gene insertion site

-30 to -1 NT upstream

LtrB intron

+1 to +15 NT downstream

Score

IS605 435|436 s

CCGATTATAGATCATCAAAAATATAGTGTA

gtgc…tcac

GAAATTATTCGCAAA

9.3

BAS4553 343|344 s

GTTCAAATGGATTATACGGATGCACGTACG

gtgc…tcac

GCTTTTTGGAAGTGT

8.4

BAS4597 1794|1795 s

AATCAATTAGATTGGGATCGAAAAGAGAAA

gtgc…tcac

GAAATAGAGACCGTT

7.3

  1. The gene chosen for intron insertion is shown in the first column with the nucleotide positions at which the intron is designed to insert at within the gene shown beside the gene name. All intron insertions are in the sense orientation relative to gene transcription and designated here with the suffix “s”. The sequence of 30 nucleotides upstream and 15 nucleotides downstream of the intron insertion site are shown together with a numeric score reflecting expected efficiency based on a learning set of Ll.Ltrb intron insertions.
Back to article page

BMC Biotechnology

ISSN: 1472-6750

Contact us