Combinatorial targeting of 2 different steps in adenoviral DNA replication by herpes simplex virus thymidine kinase and artificial microRNA expression for the inhibition of virus multiplication in the presence of ganciclovir
© Ibrišimović et al.; licensee BioMed Central Ltd. 2013
Received: 15 January 2013
Accepted: 19 June 2013
Published: 3 July 2013
Human adenoviruses are a frequent threat to immunocompromised patients, and disseminated disease is associated with severe morbidity and mortality. Current drugs are not capable of preventing all fatalities, thus indicating the need for alternative treatment strategies. Adenoviruses can be rendered susceptible to antiherpetic prodrugs such as ganciclovir (GCV), upon expression of the herpes simplex virus thymidine kinase (HSV-TK) gene in adenovirus-infected cells. Furthermore, adenoviruses are amenable to post-transcriptional gene silencing via small interfering RNAs (siRNAs) or artificial micro RNAs (amiRNAs).
In this study, we combined these 2 approaches by constructing a combinatorial gene expression cassette that comprises the HSV-TK gene and multiple copies of an amiRNA directed against the mRNA encoding the adenoviral preterminal protein (pTP). HSV-TK gene expression was controlled by the adenoviral E4 promoter, which is activated in the presence of the adenoviral E1 gene products (i.e., when adenovirus is present in the cell). When inserted into a replication-deficient (E1-, E3-deleted) adenoviral vector, this cassette effectively inhibited the replication of wild-type adenovirus in vitro. The reduction rate mediated by the combinatorial approach was higher compared to that achieved by either of the 2 approaches alone, and these obvious additive effects became most pronounced when the GCV concentration was low.
The concept presented here has the potential to aid in the inhibition of wild-type adenovirus replication. Furthermore, the combinatorial expression cassette may constitute a safeguard to potentially control unintended replication of adenoviral vectors and to prevent immune responses provoked by them.
KeywordsAdenovirus Vector RNA interference MicroRNA Ganciclovir HSV-TK
Human adenoviruses are double-stranded (ds) DNA viruses that represent a major risk for immunocompromised patients [1–3], and severe manifestations of adenoviral infections can be life-threatening. Mortality rates as high as 80% have been reported in cases of disseminated disease [4–8]. The incidence of disseminated disease is highest among hematopoietic stem cell transplant recipients, and adenoviruses belonging to species B and C are the main cause of severe adenovirus infections [3, 9–11].
Cidofovir (CDV) is the most commonly used agent for the treatment of adenovirus infections. Although the drug demonstrates clinical efficacy, its activity is not sufficient to prevent fatal outcomes in all instances [12–16], and derivatives of CDV are still being evaluated. Thus, alternative strategies to treat severe adenovirus infections have been developed. Donor lymphocyte infusion therapy, and particularly the adoptive transfer of adenovirus-specific T cells represents a promising approach for the treatment of immunocompromised patients [17, 18], but its efficacy is still under investigation.
We and others recently investigated the potential of RNA interference (RNAi)-mediated silencing of adenoviral gene expression in the control of the multiplication of adenoviruses in vitro[19–22]. RNAi-based approaches to silence viral and non-viral genes employ either the transduction of cells with short interfering RNAs (siRNAs) or the intracellular generation of short hairpin RNAs (shRNAs) and precursors of artificial miRNAs (amiRNAs), respectively, from DNA sequences introduced into those cells [23–28]. In contrast to exogenously added siRNAs, shRNAs and precursor amiRNAs must undergo intracellular processing through the RNAi pathway prior to recognizing their respective target mRNAs and eventually mediating their destruction or causing translational repression. By employing siRNAs directed against a set of adenoviral transcripts required for very different viral processes, genes essential for adenoviral DNA synthesis (i.e., those encoding the preterminal protein (pT) and the viral DNA polymerase) emerged as promising targets for the inhibition of virus multiplication . Furthermore, in a modification of the approach, an amiRNA directed against the pTP mRNA was introduced into wild-type (wt) adenovirus-infected cells via adenoviral vectors . In both approaches, the output of infectious virus progeny from infected cells could be decreased by several orders of magnitude, indicating that RNAi-based methods can, in principle, be employed to control adenovirus replication.
In a very distinct approach, we rendered adenovirus susceptible to treatment with the antiherpetic compound, ganciclovir (GCV), through the targeted expression of the herpes simplex virus thymidine kinase (HSV-TK) gene in wt Ad5-infected cells . GCV is a prodrug that requires phosphorylation by herpes viruses-encoded thymidine kinases to be efficiently converted into its active form . Once activated (i.e., when present in its tri-phosphorylated form), GCV functions as a nucleoside analog, blocking both cellular and viral DNA synthesis by competing with dGTP for incorporation into nascent DNA strands [31, 32]. The first phosphorylation step, which is efficiently carried out only by thymidine kinases encoded by herpes viruses (and not by adenoviruses or other viruses), explains the selectivity of GCV for herpes virus-infected cells . Targeted expression of HSV-TK in wt Ad5-infected cells was accomplished by inserting the HSV-TK open reading frame downstream of the Ad5 E4 promoter, whose activity is strongly increased in the presence of the Ad5 E1A gene products (i.e., in cells infected with wt adenovirus) [34, 35]. Introducing the HSV-TK expression cassette into wt Ad5-infected cells via a replication-deficient adenoviral vector lacking the E1A region strongly inhibited wt Ad5 DNA replication upon treatment of the cells with low concentrations of GCV, while no obvious effects on viability were observed in cells not infected with wt Ad5.
In the study presented here, we integrated the 2 approaches by generating adenoviral vectors that express both the HSV-TK gene from the adenoviral E4 promoter and, from a distinct expression unit, multiple copies of an amiRNA directed against the wt Ad5 pTP mRNA. We present data indicating that, upon treatment with GCV, the simultaneous expression of both cassettes in wt Ad5-infected cells results in additive anti-adenoviral effects in vitro. Moreover, we demonstrate that the additional expression of amiRNAs directed against viral pTP transcripts allows for lower levels of GCV treatment without a loss of antiadenoviral activity. Finally, we discuss how this combinatorial gene expression cassette may be used as a safeguard to potentially control unintended replication of adenoviral vectors and to prevent immune responses provoked by them.
Cell culture, virus amplification, and titer determination
HEK 293 (human embryonic kidney; ATCC CRL-1573), A549 (human epithelial lung carcinoma; ATCC CCL-185), and T-REx-293 cells (Life Technologies Austria, Vienna, Austria) were cultivated in Dulbecco’s Modified Eagle’s Medium (DMEM) with stabilized glutamine (PAA Laboratories, Pasching, Austria) and supplemented with 10% fetal bovine serum (FBS; PAA Laboratories) in a humidified 5% CO2 atmosphere at 37°C. Recombinant adenoviral vectors expressing Ad5-directed amiRNAs alone or in combination with the HSV-TK gene were amplified in T-REx-293 cells. Titers of recombinant adenoviruses expressing amiRNAs were determined on T-REx-293 cells by 50% tissue culture infective dose (TCID50) assays. Titers of wt Ad5 present in mixed virus suspensions containing both wt and recombinant virus as obtained in co-infection experiments were determined on A549 cells using the same method. All other TCID50 assays were performed with HEK 293 cells. Crude virus suspensions for titer determination were obtained by freeze-thawing the samples thrice and removing cell debris by centrifugation at 2800 rpm for 15 min.
Adenoviral vectors for the combinatorial expression of amiRNAs and HSV-TK were generated by first constructing plasmid vector versions thereof. These “entry” vectors are based on Life Technologie’s Gateway system for recombination-mediated cloning. From these entry vectors, the expression cassettes were eventually transferred into the adenoviral vector backbones via site-specific recombination. All entry vectors for combinatorial amiRNA/HSV-TK expression are based on pEE4_TK  and carry the herpes simplex virus 1 thymidine kinase gene downstream of the Ad5 E4 promoter. To generate the combinatorial vectors, the amiRNA expression cassettes were inserted into the XmnI site located downstream of the HSV-TK expression unit. The amiRNA expression cassettes comprise a CMV promoter/enhancer followed by a 2xTetO2 tetracyclin repressor binding site, and end with a BGH poly(A) site. This fragment was obtained by PCR amplification from pcDNA4/TO (Life Technologies Austria, Vienna, Austria) by using primers CMV-TO-f1(5′-TTGCATTTCGAATCTGCTTAGGGTTAGG-3′) and BGHpA-r2 (5′-CCCAGCGAATTCTTTCCGCCTCAGAAG-3′). The BclI site, located downstream of the promoter/operator region, was used for insertion of the EGFP/amiRNA cassettes, which comprise an open reading frame for EGFP and 1 or 6 copies of either the pTP-mi5 amiRNA or the universal, non-targeting amiRNA inserted into the EGFP 3′UTR. These cassettes were obtained by PCR amplification from vectors pcDNA6.2-GW/EmGFP-miR-neg (Life Technologies; carrying 1 copy of the non-targeting negative control amiRNA), pmiREx6 (; carrying 6 copies of the non-targeting negative control amiRNA), pmiRE-pTP-mi5 (; carrying 1 copy of pTP-mi5), and pmiRE-pTP-mi5x6 (; carrying 6 copies of pTP-mi5) using primers pmiRE-f2 (5′-CAAAAATGATCACTTTAAAACCATGGTGAGC-3′) and pmiRE-r2 (5′- AAGCTGTGATCAGATATCTCGAGTGCGGC-3′). In all amiRNA expression cassettes, the sequences giving rise to pre-amiRNA hairpins are flanked by sequences derived from the murine Mmu-miR-155 pri-miRNA. The final entry vectors were designated pTO-TK-mi- and pTO-TK-mi-×6 (carrying 1 or 6 copies of the non-targeting negative control amiRNA, respectively), and pTO-TK-pTP-mi5 and pTO-TK-pTP-mi5x6 (carrying 1 or 6 copies of pTP-mi5, respectively).
Restriction enzymes and DNA-modifying enzymes were purchased from Fermentas (St. Leon-Rot, Germany) or New England Biolabs (Frankfurt am Main, Germany). PCR was performed with Pwo DNA polymerase obtained from Roche Diagnostics (Vienna, Austria) or PEQLAB (Erlangen, Germany).
Nucleic acid extraction
For the extraction of circular plasmid DNA, an EasyPrep Pro Plasmid Miniprep Kit (Biozym, Oldendorf, Germany) or a HiSpeed Plasmid Midi Kit (QIAGEN, Hilden, Germany) was used. PCR products were purified using a QIAquick PCR Purification Kit (QIAGEN, Hilden, Germany), and adenoviral DNA was isolated with a QIAamp DNA Blood Mini Kit (QIAGEN, Hilden, Germany).
Virus replication experiments
For inhibition of adenovirus replication by siRNA-mediated gene silencing and concomitant HSV-TK expression/GCV treatment, 3e + 04 A549 cells were seeded into the wells of a 96-well plate and transfected with 30 nM siRNA specific for transcripts of the viral DNA polymerase (Pol-si2; sense 5′-CAACGUCUUCCAGCGUCCAACCAUA-3′, antisense 5′-UAUGGUUGGACGCUGGAAGACGUUG-3′), preterminal protein (pTP-si8; sense 5′-GAAAUUGAUUCUGUCGAACUCUCUU-3′, antisense 5′-AAGAGAGUUCGACAGAAUCAAUUUC-3′), IVa2 (IVa2-si2; sense 5′-AAAUACAGUCCAAGAUGCAUCUCAU-3′, antisense 5′-AUGAGAUGCAUCUUCGGACUGUAUUU-3′), hexon (Hex-si2; sense 5′-GAGAACUAAUGGGCCAACAAUCUAU-3′, antisense 5′-AUAGAUUGUUGGCCCAUUAGUUCUC-3′), or protease (Prot si-1; sense 5′-GAGCAGGAACUGAAAGCCAUUGUCA-3′, antisense 5′-UGACAAUGGCUUUCAGUUCCUGCUC-3′). A universal, non-targeting siRNA (Life Technologies) served as a negative control. The functionality of all siRNAs was assessed previously . At 24 h after transfection, the cells were transduced with the adenoviral vectors AdEE4-TK or AdEE4 (Figure 1) at an MOI of 100 TCID50/cell. At 24 h after transduction, the cells were infected with Ad5 at an MOI of 0.01 TCID50/cell and cultivated in the presence or absence of 1.2 μM GCV for an additional 2 days before extraction of DNA and determination of wt Ad5 genome copy numbers.
Inhibition of adenovirus replication by amiRNAs and/or HSV-TK expression/GCV treatment was assessed by seeding 3e + 04 A549 cells into the wells of 96-well plates, followed by transduction with the adenoviral vectors encoding HSV-TK and one or more amiRNA copies at an MOI of 100 TCID50/cell. After 24 h, the cells were infected with wt Ad5 at an MOI of 0.01 TCID50/cell and cultivated in the presence of GCV at concentrations ranging between 0 and 1.2 μM for up to 6 days. The cultures were subjected either to DNA isolation for determination of wt Ad5 genome copy numbers or to TCID50 analysis.
The inhibitory effect of the HSV-TK/amiRNA expression cassette on the replication of vector AdTO-TK-pTP-mi5x6 was determined by transducing 1.2e + 05 T-REx-293 cells with the vector at an MOI of 0.01 TCID50/cell. At the time of transduction, amiRNA expression was induced by adding 1 μg/ml doxycycline to the medium, and GCV was added at concentrations ranging between 0 and 1.2 μM. At 48 h after transduction, the cultures were subjected to DNA isolation and determination of vector copy numbers by qPCR.
Determination of adenovirus genome copy numbers
Determination of wt Ad5 DNA levels was performed by qPCR using a TaqMan primer/probe set specific for the E1A gene (E1A-fwd 5′-GACGGCCCCCGAAGATC-3′, E1A-rev 5′-TCCTGCACCGCCAACATT-3′, and E1A-p 5′-CGAGGAGGCGGTTTCGCAGA-3′). For the determination of DNA levels of recombinant adenoviral vectors, a TaqMan primer/probe set specific for the hexon gene was used (hexon-fwd 5′- CACTCATATTTCTTACATGCCCACTATT-3′, hexon-rev 5′-GGCCTGTTGGGCATAGATTG-3′, hexon-probe 5′-AGGAAGGTAACTCACGAGAACTAATGGGCCA-3′). Adenovirus genome copy numbers were determined using serial dilutions of an adenoviral reference DNA as a standard.
EGFP expression rates were determined by FACS analysis. Cells transduced with EGFP-expressing adenoviruses were harvested by trypsinization, resuspended in normal cell culture medium, and pelleted by centrifugation at 1200 rpm for 5 min. Thereafter, cells were washed once with phosphate buffered saline (PBS) and fixed with 1% formaldehyde in PBS. Samples were analyzed with a FACS Calibur analyzer (Becton Dickinson, Heidelberg, Germany), and percentages of fluorescent cells and mean fluorescence intensities (MFIs) were calculated.
All the data are expressed as mean ± standard deviation (SD). In cases in which the dataset consisted of only 2 groups of samples, Student’s t-test was applied to test for statistical significance. For the analysis of larger datasets, one-way ANOVA, corrected with Bonferroni´s post-hoc test, was applied. A p-value less than 0.05 was considered statistically significant.
Adenovirus-directed siRNAs increase the HSV-TK/GCV-mediated anti-adenoviral effect
We have previously shown that siRNAs or adenoviral vector-encoded amiRNAs targeting viral mRNAs coding for essential viral DNA synthesis components (i.e., the viral DNA polymerase and pTP, respectively) can inhibit wt Ad5 replication in vitro[21, 22]. We have also demonstrated that the targeted expression of HSV-TK in wt Ad5-infected cells renders adenovirus amenable to inhibition by GCV, through the suppression of viral DNA synthesis . Thus, it is conceivable that a combination of the 2 approaches can lead to additive effects.
Combined HSV-TK and amiRNA expression increases the anti-adenoviral effect in the presence of GCV
These results prompted us to generate a combinatorial adenoviral vector harboring the HSV-TK expression unit, such as that present on AdEE4-TK, and an amiRNA expression cassette, as found in AdTO-pTP-mi5. In our previous study , an amiRNA (pTP-mi5) targeting the Ad5 pTP mRNA was identified as the most potent amiRNA for inhibition of wt Ad5 DNA replication in vitro. Thus, in the present study, we merged the adenoviral HSV-TK expression vector, AdEE4-TK, with the adenoviral pTP-mi5 expression vector, AdTO-pTP-mi5, to create the adenoviral vector AdTO-TK-pTP-mi5 (Figure 1). A corresponding negative control vector (AdTO-TK-mi-) carrying an expression cassette for a negative control amiRNA instead of pTP-mi5 was also constructed. We decided to use replication-deficient adenoviral vectors for the combined HSV-TK/amiRNA expression and delivery, because this type of vector may prove advantageous in an envisioned therapeutic application. Because of the shared organ tropism of the adenoviral vector and the wt virus, such a vector may ensure the delivery of the expression cassettes into those cells that are also the preferred targets of the wt virus.
Multicopy amiRNA/HSV-TK expression inhibits wt adenovirus replication even at very low GCV concentrations
The combinatorial HSV-TK/amiRNA expression cassette inhibits adenoviral vector replication
We previously demonstrated the inhibition of adenovirus replication both by RNAi-based methods and through the targeted expression of HSV-TK in adenovirus-infected cells with concomitant GCV treatment. Both approaches targeted viral DNA replication, albeit in different ways. While the siRNAs/amiRNAs decreased the number of functional viral proteins that are needed for the initiation (pTP) or progression of viral DNA replication (DNA polymerase), GCV-ppp acted downstream of these steps in a functionally different way (i.e., as a nucleoside analog by preventing the DNA polymerization process). CDV is mechanistically related to GCV-ppp since it blocks the same step during virus replication , and we have demonstrated that the expression of a pTP RNA-targeting amiRNA in adenovirus-infected cells and concomitant treatment with CDV leads to additive inhibitory effects . Thus, it was conceivable that a combination of pTP gene silencing via an amiRNA and HSV-TK expression/GCV treatment can result in a similar effect. This assumption was supported by results demonstrating that siRNAs targeting viral transcripts required for DNA replication increased the HSV-TK/GCV-mediated effect (Figure 2). In these experiments, we did not only include the siRNAs with the highest previously proven inhibitory effect on adenoviral DNA replication, but also the ones that had resulted in poor antiviral effects in our previous study (i.e., the hexon and viral protease RNA-targeting siRNAs). We hypothesized that a strong inhibition of viral DNA replication by HSV-TK expression/GCV treatment (which is more effective than that mediated by viral DNA polymerase or pTP mRNA-targeting siRNAs) should decrease viral DNA genome copy numbers and, consequently, hexon and protease gene copy numbers. This, in turn, should result in a decrease in the otherwise vast amounts of hexon and protease transcripts present in adenovirus-infected cells, which may allow the siRNAs to silence their respective target genes more effectively. However, similar to our previous study, the hexon and protease RNA-targeting siRNAs were relatively poor inhibitors of virus multiplication under these conditions. These results reflect those obtained in experiments in which we inhibited viral DNA synthesis by siRNAs, but were unable to further increase the overall antiviral effect by also targeting the hexon and protease transcripts . Thus, the data presented here support our previous observations that argue against a concomitant targeting of different viral processes (e.g., viral DNA synthesis and capsid generation) as the method of choice to inhibit adenovirus multiplication but, rather, for the targeting of only a single process such as viral DNA synthesis at different steps.
Thus, it was conclusive to incorporate the individual modules for HSV-TK and pTP-mi5 expression into one common vector to concomitantly target adenoviral DNA synthesis at 2 different points. The combinatorial approach revealed an increase in the overall anti-adenoviral effect; however, this effect was modest when we inserted only one copy of the pTP-mi5 sequence into the vector (Figure 4). Because concatemerization of identical amiRNA sequences has been shown to increase knockdown rates [40, 41], and concatemerization of pTP-mi5 has also previously resulted in enhanced silencing of Ad5 pTP , we added more copies to the vector. We did not observe any detrimental effects on cell viability that could potentially occur due to the higher number of amiRNAs potentially competing with endogenous miRNAs. In case of in vivo testing of this and related vectors, this point would have to be carefully addressed, though. The increased additive effects of combined HSV-TK/amiRNA expression obtained with this final vector were most pronounced when GCV was applied at very low concentrations (0.15 μM) (Figure 5). At these low concentrations, inhibition of adenoviral replication mediated by HSV-TK expression alone became marginal (Figure 5A); however, it was restored to normal levels upon concomitant knockdown of pTP gene expression by pTP-mi5. This observed effect is conclusive because, at very low concentrations, GCV-ppp needed for the blockage of DNA polymerization is expected to become limiting when, at the same time, high numbers of viral DNA replication complexes work in parallel to synthesize high numbers of viral DNA molecules. In contrast, the incorporation rate of GCV-ppp into nascent viral DNA strands should increase when only a few viral DNA molecules are generated.
In summary, our data suggest that wt adenovirus DNA replication can, in principle, be blocked most effectively by targeting 2 independent steps needed for viral DNA replication, namely (i) the formation of the initiation complex, and (ii) the actual DNA polymerization step. Targeting of these mechanistically different steps may not be restricted to the way we accomplished it, but may also prove a useful strategy when aiming to inhibit adenoviral DNA replication by novel “conventional” drugs or small-molecule inhibitors. In general, our strategy does not allow to cure wild-type virus-infected cells from the infection, and it cannot prevent them from cell death. However, it can effectively decrease the output of infectious virus progeny from these cells, and consequently prevent virus spreading.
In an envisioned therapeutic scenario, the delivery of anti-adenoviral amiRNAs, via a replication-deficient adenoviral vector, may have several unique advantages. For example, it may allow for the amplification of amiRNA and HSV-TK expression cassette copy numbers upon exposure of the recombinant virus to the wt virus due to the initiation of vector replication. This transcomplementation effect has already been demonstrated earlier for the pTP-mi5 amiRNA expression cassette in vitro. In addition, this effect may ensure a constant supply of recombinant vector as long as wt adenovirus is present. Moreover, due to the shared organ tropism of the adenoviral vector and its wt counterpart, delivery via an adenovirus-based vector may also permit the directing of the vector predominantly to those cells and organs that are also the preferred targets of the wt virus. Among those is the liver which functions as a major virion multiplicator during adenovirus infection and is readily transduced by adenoviral vectors . Thus, it is conceivable, that strategies as the one presented here are able to reduce the output of infectious wt virus at least from this particular organ, and, consequently, inhibit spreading of the virus throughout the body. Because the immune response against the wild-type virus is heavily impaired or inexistent in immunocompromised patients, problems that can occur due to the elimination of adenoviral vectors by the immune system should be less pronounced or absent in such an envisioned scenario. Alternatively, topical treatment of localized adenovirus infection, e.g., of the eye, where high local vector concentrations can be achieved, is conceivable for immunocompetent patients as well. Adenovirus-mediated epidemic keratoconjunctivitis (EKC) is associated with significant morbidity and possible long term consequences on visual acuity, and thus causes considerable economic losses.
Adenoviral vectors are among the most commonly used vectors for the delivery of genetic information in vivo. In most cases, replication-deficient vectors whose E1 and E3 genes are deleted are employed. In theory, no viral genes should be expressed from these vectors. Leaky expression of viral genes from replication-deficient viral vectors, however, is known to occur due to the presence of binding sites for certain cellular transcription factors in adenoviral promoters . This background expression from both early and late promoters can result in toxicity and immunity against adenoviral proteins [44–48] and, consequently, to short-lived transgene expression. The amiRNA generated from the combinatorial HSV-TK/amiRNA expression cassette, when included in such vectors, may potentially prevent leaky pTP expression from the adenoviral E2B promoter. It may, thus, inhibit any potential low-level replication and, consequently, late gene expression (which is dependent on viral DNA replication), thereby preventing the generation of highly immunogenic late gene products such as the hexon and fiber proteins. In fact, deletions within the E2B region that comprises the viral DNA polymerase and pTP genes lead to decreased downstream gene expression in E1-deleted adenoviral vectors [49, 50], resulting in extended transgene expression and reduced liver toxicity [50, 51].
The HSV-TK expression unit of the cassette, in conjunction with the amiRNA expression unit, may also help to bring any unforeseen high-level replication of adenoviral vectors under control, should they turn into replication-competent versions (i.e., upon unintended recombination with a wt virus). The elimination upon treatment with GCV would only affect the rearranged vector and not the parental vector because the latter would not promote the synthesis of significant amounts of HSV-TK. Thus, the combinatorial gene expression cassette presented here (or similar versions following the same principle) may also be considered for evaluation as safeguards for certain adenoviral vectors intended to be used in gene therapy studies.
In summary, the results presented in this study demonstrate that combined expression of HSV-TK and an amiRNA targeting the adenoviral pTP mRNA can effectively inhibit wt adenovirus replication in the presence of the antiherpetic prodrug GCV. The enhanced inhibition rate accomplished with the combinatorial gene expression cassette can most likely be attributed to the targeting of 2 different steps in adenoviral DNA synthesis. This effect mediated by the expression cassette may not only be harnessed to inhibit wt adenovirus replication but also to prevent the replication of certain adenoviral vectors commonly used in gene therapy studies.
This work was partially supported by the Austrian Science Fund through grant L665-B13 and by the Austrian National Bank through grant 12814.
- Echavarria M: Adenoviruses in immunocompromised hosts. Clin Microbiol Rev. 2008, 21 (4): 704-715. 10.1128/CMR.00052-07.View ArticleGoogle Scholar
- Ison MG: Adenovirus infections in transplant recipients. Clin Infect Dis. 2006, 43 (3): 331-339. 10.1086/505498.View ArticleGoogle Scholar
- Kojaoghlanian T, Flomenberg P, Horwitz MS: The impact of adenovirus infection on the immunocompromised host. Rev Med Virol. 2003, 13 (3): 155-171. 10.1002/rmv.386.View ArticleGoogle Scholar
- Blanke C, Clark C, Broun ER, Tricot G, Cunningham I, Cornetta K, Hedderman A, Hromas R: Evolving pathogens in allogeneic bone marrow transplantation: increased fatal adenoviral infections. Am J Med. 1995, 99 (3): 326-328. 10.1016/S0002-9343(99)80169-7.View ArticleGoogle Scholar
- Hale GA, Heslop HE, Krance RA, Brenner MA, Jayawardene D, Srivastava DK, Patrick CC: Adenovirus infection after pediatric bone marrow transplantation. Bone Marrow Transplant. 1999, 23 (3): 277-282. 10.1038/sj.bmt.1701563.View ArticleGoogle Scholar
- Howard DS, Phillips IG, Reece DE, Munn RK, Henslee-Downey J, Pittard M, Barker M, Pomeroy C: Adenovirus infections in hematopoietic stem cell transplant recipients. Clin Infect Dis. 1999, 29 (6): 1494-1501. 10.1086/313514.View ArticleGoogle Scholar
- Lion T, Baumgartinger R, Watzinger F, Matthes-Martin S, Suda M, Preuner S, Futterknecht B, Lawitschka A, Peters C, Potschger U, et al: Molecular monitoring of adenovirus in peripheral blood after allogeneic bone marrow transplantation permits early diagnosis of disseminated disease. Blood. 2003, 102 (3): 1114-1120. 10.1182/blood-2002-07-2152.View ArticleGoogle Scholar
- Munoz FM, Piedra PA, Demmler GJ: Disseminated adenovirus disease in immunocompromised and immunocompetent children. Clin Infect Dis. 1998, 27 (5): 1194-1200. 10.1086/514978.View ArticleGoogle Scholar
- Ebner K, Rauch M, Preuner S, Lion T: Typing of human adenoviruses in specimens from immunosuppressed patients by PCR-fragment length analysis and real-time quantitative PCR. J Clin Microbiol. 2006, 44 (8): 2808-2815. 10.1128/JCM.00048-06.View ArticleGoogle Scholar
- Ebner K, Suda M, Watzinger F, Lion T: Molecular detection and quantitative analysis of the entire spectrum of human adenoviruses by a two-reaction real-time PCR assay. J Clin Microbiol. 2005, 43 (7): 3049-3053. 10.1128/JCM.43.7.3049-3053.2005.View ArticleGoogle Scholar
- Lion T, Kosulin K, Landlinger C, Rauch M, Preuner S, Jugovic D, Potschger U, Lawitschka A, Peters C, Fritsch G, et al: Monitoring of adenovirus load in stool by real-time PCR permits early detection of impending invasive infection in patients after allogeneic stem cell transplantation. Leukemia. 2010, 24 (4): 706-714. 10.1038/leu.2010.4.View ArticleGoogle Scholar
- Lenaerts L, De Clercq E, Naesens L: Clinical features and treatment of adenovirus infections. Rev Med Virol. 2008, 18 (6): 357-374. 10.1002/rmv.589.View ArticleGoogle Scholar
- Lindemans CA, Leen AM, Boelens JJ: How I treat adenovirus in hematopoietic stem cell transplant recipients. Blood. 2010, 116 (25): 5476-5485. 10.1182/blood-2010-04-259291.View ArticleGoogle Scholar
- Ljungman P, Ribaud P, Eyrich M, Matthes-Martin S, Einsele H, Bleakley M, Machaczka M, Bierings M, Bosi A, Gratecos N, et al: Cidofovir for adenovirus infections after allogeneic hematopoietic stem cell transplantation: a survey by the infectious diseases working party of the European group for blood and marrow transplantation. Bone Marrow Transplant. 2003, 31 (6): 481-486. 10.1038/sj.bmt.1703798.View ArticleGoogle Scholar
- Symeonidis N, Jakubowski A, Pierre-Louis S, Jaffe D, Pamer E, Sepkowitz K, O’Reilly RJ, Papanicolaou GA: Invasive adenoviral infections in T-cell-depleted allogeneic hematopoietic stem cell transplantation: high mortality in the era of cidofovir. Transpl Infect Dis. 2007, 9 (2): 108-113. 10.1111/j.1399-3062.2006.00184.x.View ArticleGoogle Scholar
- Yusuf U, Hale GA, Carr J, Gu Z, Benaim E, Woodard P, Kasow KA, Horwitz EM, Leung W, Srivastava DK, et al: Cidofovir for the treatment of adenoviral infection in pediatric hematopoietic stem cell transplant patients. Transplantation. 2006, 81 (10): 1398-1404. 10.1097/01.tp.0000209195.95115.8e.View ArticleGoogle Scholar
- Feuchtinger T, Matthes-Martin S, Richard C, Lion T, Fuhrer M, Hamprecht K, Handgretinger R, Peters C, Schuster FR, Beck R, et al: Safe adoptive transfer of virus-specific T-cell immunity for the treatment of systemic adenovirus infection after allogeneic stem cell transplantation. Br J Haematol. 2006, 134 (1): 64-76. 10.1111/j.1365-2141.2006.06108.x.View ArticleGoogle Scholar
- Leen AM, Christin A, Myers GD, Liu H, Cruz CR, Hanley PJ, Kennedy-Nasser AA, Leung KS, Gee AP, Krance RA, et al: Cytotoxic T lymphocyte therapy with donor T cells prevents and treats adenovirus and Epstein-Barr virus infections after haploidentical and matched unrelated stem cell transplantation. Blood. 2009, 114 (19): 4283-4292. 10.1182/blood-2009-07-232454.View ArticleGoogle Scholar
- Chung YS, Kim MK, Lee WJ, Kang C: Silencing E1A mRNA by RNA interference inhibits adenovirus replication. Arch Virol. 2007, 152 (7): 1305-1314. 10.1007/s00705-007-0951-z.View ArticleGoogle Scholar
- Eckstein A, Grossl T, Geisler A, Wang X, Pinkert S, Pozzuto T, Schwer C, Kurreck J, Weger S, Vetter R, et al: Inhibition of adenovirus infections by siRNA-mediated silencing of early and late adenoviral gene functions. Antiviral Res. 2010, 88 (1): 86-94. 10.1016/j.antiviral.2010.08.002.View ArticleGoogle Scholar
- Ibrisimovic M, Kneidinger D, Lion T, Klein R: An adenoviral vector-based expression and delivery system for the inhibition of wild-type adenovirus replication by artificial microRNAs. Antiviral Res. 2012, 97 (1): 10-23.View ArticleGoogle Scholar
- Kneidinger D, Ibrisimovic M, Lion T, Klein R: Inhibition of adenovirus multiplication by short interfering RNAs directly or indirectly targeting the viral DNA replication machinery. Antiviral Res. 2012, 94 (3): 195-207. 10.1016/j.antiviral.2012.03.011.View ArticleGoogle Scholar
- Elbashir SM, Harborth J, Lendeckel W, Yalcin A, Weber K, Tuschl T: Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature. 2001, 411 (6836): 494-498. 10.1038/35078107.View ArticleGoogle Scholar
- Cullen BR: Derivation and function of small interfering RNAs and microRNAs. Virus Res. 2004, 102 (1): 3-9. 10.1016/j.virusres.2004.01.009.View ArticleGoogle Scholar
- Burnett JC, Rossi JJ: RNA-based therapeutics: current progress and future prospects. Chem Biol. 2012, 19 (1): 60-71. 10.1016/j.chembiol.2011.12.008.View ArticleGoogle Scholar
- Zeng Y, Wagner EJ, Cullen BR: Both natural and designed micro RNAs can inhibit the expression of cognate mRNAs when expressed in human cells. Molecular cell. 2002, 9 (6): 1327-1333. 10.1016/S1097-2765(02)00541-5.View ArticleGoogle Scholar
- Davidson BL, McCray PB: Current prospects for RNA interference-based therapies. Nat Rev Genet. 2011, 12 (5): 329-340. 10.1038/nrg2968.View ArticleGoogle Scholar
- Huntzinger E, Izaurralde E: Gene silencing by microRNAs: contributions of translational repression and mRNA decay. Nat Rev Genet. 2011, 12 (2): 99-110. 10.1038/nrg2936.View ArticleGoogle Scholar
- Ibrisimovic M, Nagl U, Kneidinger D, Rauch M, Lion T, Klein R: Targeted expression of herpes simplex virus thymidine kinase in adenovirus-infected cells reduces virus titers upon treatment with ganciclovir in vitro. J Gene Med. 2012, 14 (1): 3-19.View ArticleGoogle Scholar
- Faulds D, Heel RC: Ganciclovir. A review of its antiviral activity, pharmacokinetic properties and therapeutic efficacy in cytomegalovirus infections. Drugs. 1990, 39 (4): 597-638. 10.2165/00003495-199039040-00008.View ArticleGoogle Scholar
- Ilsley DD, Lee SH, Miller WH, Kuchta RD: Acyclic guanosine analogs inhibit DNA polymerases alpha, delta, and epsilon with very different potencies and have unique mechanisms of action. Biochemistry. 1995, 34 (8): 2504-2510. 10.1021/bi00008a014.View ArticleGoogle Scholar
- Golankiewicz B, Ostrowski T: Tricyclic nucleoside analogues as antiherpes agents. Antiviral Res. 2006, 71 (2–3): 134-140.View ArticleGoogle Scholar
- Elion GB, Furman PA, Fyfe JA, de Miranda P, Beauchamp L, Schaeffer HJ: Selectivity of action of an antiherpetic agent, 9-(2-hydroxyethoxymethyl) guanine. Proc Natl Acad Sci U S A. 1977, 74 (12): 5716-5720. 10.1073/pnas.74.12.5716.View ArticleGoogle Scholar
- Berk AJ: Recent lessons in gene expression, cell cycle control, and cell biology from adenovirus. Oncogene. 2005, 24 (52): 7673-7685. 10.1038/sj.onc.1209040.View ArticleGoogle Scholar
- Liu F, Green MR: Promoter targeting by adenovirus E1a through interaction with different cellular DNA-binding domains. Nature. 1994, 368 (6471): 520-525. 10.1038/368520a0.View ArticleGoogle Scholar
- Asano-Mori Y, Kanda Y, Oshima K, Watanabe T, Shoda E, Motokura T, Kurokawa M, Chiba S: Pharmacokinetics of ganciclovir in haematopoietic stem cell transplantation recipients with or without renal impairment. J Antimicrob Chemother. 2006, 57 (5): 1004-1007. 10.1093/jac/dkl089.View ArticleGoogle Scholar
- Caldes A, Colom H, Armendariz Y, Garrido MJ, Troconiz IF, Gil-Vernet S, Lloberas N, Pou L, Peraire C, Grinyo JM: Population pharmacokinetics of ganciclovir after intravenous ganciclovir and oral valganciclovir administration in solid organ transplant patients infected with cytomegalovirus. Antimicrob Agents Chemother. 2009, 53 (11): 4816-4824. 10.1128/AAC.00085-09.View ArticleGoogle Scholar
- Frenkel LM, Capparelli EV, Dankner WM, Xu J, Smith IL, Ballow A, Culnane M, Read JS, Thompson M, Mohan KM, et al: Oral ganciclovir in children: pharmacokinetics, safety, tolerance, and antiviral effects. The Pediatric AIDS Clinical Trials Group. J Infect Dis. 2000, 182 (6): 1616-1624. 10.1086/317600.View ArticleGoogle Scholar
- Cundy KC: Clinical pharmacokinetics of the antiviral nucleotide analogues cidofovir and adefovir. Clin Pharmacokinet. 1999, 36 (2): 127-143. 10.2165/00003088-199936020-00004.View ArticleGoogle Scholar
- Chung KH, Hart CC, Al-Bassam S, Avery A, Taylor J, Patel PD, Vojtek AB, Turner DL: Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155. Nucleic Acids Res. 2006, 34 (7): e53-10.1093/nar/gkl143.View ArticleGoogle Scholar
- Wu Z, Xue Y, Wang B, Du J, Jin Q: Broad-spectrum antiviral activity of RNA interference against four genotypes of Japanese encephalitis virus based on single microRNA polycistrons. PLoS One. 2011, 6 (10): e26304-10.1371/journal.pone.0026304.View ArticleGoogle Scholar
- Khare R, Chen CY, Weaver EA, Barry MA: Advances and future challenges in adenoviral vector pharmacology and targeting. Current gene therapy. 2011, 11 (4): 241-258. 10.2174/156652311796150363.View ArticleGoogle Scholar
- Jones NC, Rigby PW, Ziff EB: Trans-acting protein factors and the regulation of eukaryotic transcription: lessons from studies on DNA tumor viruses. Genes Dev. 1988, 2 (3): 267-281. 10.1101/gad.2.3.267.View ArticleGoogle Scholar
- Yang Y, Nunes FA, Berencsi K, Gonczol E, Engelhardt JF, Wilson JM: Inactivation of E2a in recombinant adenoviruses improves the prospect for gene therapy in cystic fibrosis. Nat Genet. 1994, 7 (3): 362-369. 10.1038/ng0794-362.View ArticleGoogle Scholar
- Yang Y, Nunes FA, Berencsi K, Furth EE, Gonczol E, Wilson JM: Cellular immunity to viral antigens limits E1-deleted adenoviruses for gene therapy. Proc Natl Acad Sci U S A. 1994, 91 (10): 4407-4411. 10.1073/pnas.91.10.4407.View ArticleGoogle Scholar
- Engelhardt JF, Litzky L, Wilson JM: Prolonged transgene expression in cotton rat lung with recombinant adenoviruses defective in E2a. Hum Gene Ther. 1994, 5 (10): 1217-1229. 10.1089/hum.1994.5.10-1217.View ArticleGoogle Scholar
- Lieber A, He CY, Meuse L, Himeda C, Wilson C, Kay MA: Inhibition of NF-kappaB activation in combination with bcl-2 expression allows for persistence of first-generation adenovirus vectors in the mouse liver. J Virol. 1998, 72 (11): 9267-9277.Google Scholar
- Lieber A, He CY, Meuse L, Schowalter D, Kirillova I, Winther B, Kay MA: The role of Kupffer cell activation and viral gene expression in early liver toxicity after infusion of recombinant adenovirus vectors. J Virol. 1997, 71 (11): 8798-8807.Google Scholar
- Amalfitano A, Hauser MA, Hu H, Serra D, Begy CR, Chamberlain JS: Production and characterization of improved adenovirus vectors with the E1, E2b, and E3 genes deleted. J Virol. 1998, 72 (2): 926-933.Google Scholar
- Everett RS, Hodges BL, Ding EY, Xu F, Serra D, Amalfitano A: Liver toxicities typically induced by first-generation adenoviral vectors can be reduced by use of E1, E2b-deleted adenoviral vectors. Hum Gene Ther. 2003, 14 (18): 1715-1726. 10.1089/104303403322611737.View ArticleGoogle Scholar
- Hodges BL, Serra D, Hu H, Begy CA, Chamberlain JS, Amalfitano A: Multiply deleted [E1, polymerase-, and pTP-] adenovirus vector persists despite deletion of the preterminal protein. J Gene Med. 2000, 2 (4): 250-259. 10.1002/1521-2254(200007/08)2:4<250::AID-JGM113>3.0.CO;2-3.View ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.