Figure 4

Binding activity of TfRscFv-GAL4 fusion protein to gastric cancer and breast cancer in TMA. 140 gastric cancer tissue and 112 breast cancer tissue were embeded into TMA, dissect into 5μm and placed on one slide to make tissue microarray sections, TfRscFv-GAL4 single-chain antibodies were used to bind to the TMA sections (SP: × 400) A-B. Mouse anti-GAL4 antibody was used to bind the single-chain antibody, HRP conjugated goat anti-mouse antibody and DAB were used for detection of TfR protein expression. Parental mouse anti-TfR antibody was used as positive control (SP: × 400) C-D. The typical immunohistology results of positive binding activity with tumor tissues have been shown in A-D (SP: × 400). Both anti-GLA4 antibody and normal mouse IgG were used as negative control were typical immunohistology results of negative binding activity with tumor tissues (SP: × 400) E-H. Table 1 showed the statistic data of all tissue in immunohistology results.