Figure 1

Construction strategy of TfRscFv-GAL4 prokaryotic expression vector. The prokaryotic expression vector pET28-a was used as the backbone, the heavy chain (VH) and the light chain (VL) of ScFv was connected with a linker (VH-linker-VL), the His-tag of GAL4 DNA Binding Domain(GAL4-DBD) was fused to the 3’-end of the VH-linker-VL sequence to express the tag conjugated to C-terminal of the single-chain antibody. The whole sequence was cloned into the NcoI-NotI restriction site to get a prokaryotic expression vector of TfRscFv-GAL4-pET. The steps taken in the construction of each plasmid are detailed in Methods.