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Table 1 Primers used in PCR and RT-PCR analyses

From: Enhanced recombinant protein production and differential expression of molecular chaperones in sf-caspase-1-repressed stable cells after baculovirus infection

Gene Protein/product Predicted location Description Primer sequence PCR cycle Reference
Sf-casp1 Sf-casp1 dsRNA nucleus/cytosol pIBdsCasp DNA, forward 5-GATATGGAGAAGATCTGGCAC-3 35 [25]
5-GTGGGGCAGGGCGTAGCC-3
pIBdsCasp DNA, reverse
Sf-β-actin β-Actin cytosol Sf-β-actin DNA/mRNA, forward 5-CAATCTGTCACCTTGGCA-3 25 [25]
5-GACAATACAAACTAAGATTTA-3
Sf-β-actin DNA/mRNA, reverse
Sf-casp1 Sf-Caspase-1 cytosol Sf-casp1 mRNA, forward 5-TGTCAAACACCTTTTATG-3 25 [25]
5-TATTATGACACATGGGCA-3
Sf-casp1 mRNA, reverse
atx1 Copper chaperone cytosol atx1 mRNA, forward 5-ATGTCATCTACACACATTTT-3 25 [29]
atx1 mRNA, reverse
5-CTATTGTGTGCCAACATAAG-3
cypA Cyclophilin A (CypA) cytosol cypA mRNA, forward 5-ATGGCTTTACCCCGAGTTT-3 25 [29]
5-TGGTTTGTGAAGTCACCTCCT-3
cypA mRNA, reverse
cypB Cyclophilin B (CypB) secreted/ER cypB mRNA, forward 5-GCGCTTTCGCAGCAGCTCTT-3 25 [29]
5-AGCCCTGGCCTTCTGGTTT-3
cypB mRNA, reverse
hsc70 Heat shock cognate ER hsc70 mRNA, forward 5-ATGATCAAAATGCGGTGGA-3 25 [30]
70 (GRP78-like) 5-AGCCACATATGAGGGAGTGAT-3
hsc70 mRNA, reverse
hsp90 Heat shock protein ER hsp90 mRNA, forward 5-ATGCCCGAAGAAATGCAG-3 25 [31]
90 (HSP90)
hsp90 mRNA, reverse
5-TGTACAGCTCCTTGCCGCT-3
calr Calreticulin (CRT) cytosol calr mRNA, forward 5-ATTCTATTCGTGGCCAGTCC-3 25 [29]
5-GAACTTTCCAGCAGTCAGCTT-3
calr mRNA, reverse
pdi Protein disulfide ER pdi mRNA, forward 5-GCCATTGACGCTGACGAA-3 25 [29]
  isomerase (PDI)   pdi mRNA, reverse 5-CCTCGCTCAACAAGTGCTGT-3   
  1. ER, endoplasmic reticulum.
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