Table 1 Primers used in PCR and RT-PCR analyses
Gene | Protein/product | Predicted location | Description | Primer sequence | PCR cycle | Reference |
|---|---|---|---|---|---|---|
Sf-casp1 | Sf-casp1 dsRNA | nucleus/cytosol | pIBdsCasp DNA, forward | 5′-GATATGGAGAAGATCTGGCAC-3′ | 35 | [25] |
5′-GTGGGGCAGGGCGTAGCC-3′ | ||||||
pIBdsCasp DNA, reverse | ||||||
Sf-β-actin | β-Actin | cytosol | Sf-β-actin DNA/mRNA, forward | 5′-CAATCTGTCACCTTGGCA-3′ | 25 | [25] |
5′-GACAATACAAACTAAGATTTA-3′ | ||||||
Sf-β-actin DNA/mRNA, reverse | ||||||
Sf-casp1 | Sf-Caspase-1 | cytosol | Sf-casp1 mRNA, forward | 5′-TGTCAAACACCTTTTATG-3′ | 25 | [25] |
5′-TATTATGACACATGGGCA-3′ | ||||||
Sf-casp1 mRNA, reverse | ||||||
atx1 | Copper chaperone | cytosol | atx1 mRNA, forward | 5′-ATGTCATCTACACACATTTT-3′ | 25 | [29] |
atx1 mRNA, reverse | ||||||
5′-CTATTGTGTGCCAACATAAG-3′ | ||||||
cypA | Cyclophilin A (CypA) | cytosol | cypA mRNA, forward | 5′-ATGGCTTTACCCCGAGTTT-3′ | 25 | [29] |
5′-TGGTTTGTGAAGTCACCTCCT-3′ | ||||||
cypA mRNA, reverse | ||||||
cypB | Cyclophilin B (CypB) | secreted/ER | cypB mRNA, forward | 5′-GCGCTTTCGCAGCAGCTCTT-3′ | 25 | [29] |
5′-AGCCCTGGCCTTCTGGTTT-3′ | ||||||
cypB mRNA, reverse | ||||||
hsc70 | Heat shock cognate | ER | hsc70 mRNA, forward | 5′-ATGATCAAAATGCGGTGGA-3′ | 25 | [30] |
70 (GRP78-like) | 5′-AGCCACATATGAGGGAGTGAT-3′ | |||||
hsc70 mRNA, reverse | ||||||
hsp90 | Heat shock protein | ER | hsp90 mRNA, forward | 5′-ATGCCCGAAGAAATGCAG-3′ | 25 | [31] |
90 (HSP90) | ||||||
hsp90 mRNA, reverse | ||||||
5′-TGTACAGCTCCTTGCCGCT-3′ | ||||||
calr | Calreticulin (CRT) | cytosol | calr mRNA, forward | 5′-ATTCTATTCGTGGCCAGTCC-3′ | 25 | [29] |
5′-GAACTTTCCAGCAGTCAGCTT-3′ | ||||||
calr mRNA, reverse | ||||||
pdi | Protein disulfide | ER | pdi mRNA, forward | 5′-GCCATTGACGCTGACGAA-3′ | 25 | [29] |
|  | isomerase (PDI) |  | pdi mRNA, reverse | 5′-CCTCGCTCAACAAGTGCTGT-3′ |  |  |