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Figure 3 | BMC Biotechnology

Figure 3

From: Enhanced recombinant protein production and differential expression of molecular chaperones in sf-caspase-1-repressed stable cells after baculovirus infection

Figure 3

Genomic DNA PCR (A) and RT-PCR (B) analysis in Sf9/pIBdsCasp-1 and Sf9/pIBdsCasp-2 cells. Genomic DNA and total RNA of the cells (Sf9, Sf9/pIB, Sf9/pIBdsCasp-1 and Sf9/pIBdsCasp-2) were extracted and analyzed by PCR and RT-PCR, respectively. The corresponding specific primer sequences and PCR cycles are presented in Table 1. pIB, Sf9/pIB cells; dsCasp-1, Sf9/pIBdsCasp-1 cells; dsCasp-2, Sf9/pIBdsCasp-2 cells.

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