Development of an improved polykaryon-based influenza virus rescue system

BMC Biotechnology

Table 1 Yields (in p.f.u./mL) from cells transfected with partial complements of the eight PR8 rescue plasmids

This table shows the viral yields (p.f.u./mL) from cells transfected with the eight PR8 rescue plasmids split into separate batches at the time of transfection and brought together on the next day.
¹ In the "4 + 4" experiment, one batch of cells was transfected with plasmids encoding segments 1, 2, 3, 5, while a complementary batch of cells was transfected with plasmids encoding segments 4, 6, 7, 8. In the "7 + 1" experiment, one batch was transfected with plasmids encoding segments 1, 3, 4, 5, 6, 7, 8 and the complementary batch was transfected with segment 2. The cells from complementary batches were dislodged and brought together at day 1 post transfection. These experiments consisted of triplicate transfections for each condition (Repeats 1 to 3).
² In addition to the transfection with influenza plasmids, cells were transfected with either the Env-expressing or the empty B1 plasmid.

ISSN: 1472-6750