Cell cultivation and ΔNS1 H1N1 Influenza virus amplification in 3 L stirred tank bioreactor using EX-CELL Vero SFM with 3 g/l Cytodex 1, MOI of 0.001 and trypsin concentration of 10 μg/ml. (A) Cell growth profile and virus amplification of representative bioreactor run. Error bars indicate the standard error of triplicate measurements. (B) Metabolite profiles of glucose, lactate, glutamine and ammonium, before and after infection. (C) Comparison of max virus titres between infection at M.O.I 0.001 and 0.01 carried out in 3 L bioreactor scale. Log2 HAU is represented as filled bars and non-filled bar indicates virus titre in Log10 TCID50/ml. Error bars indicate the standard deviation of triplicate bioreactor runs.