A: Circular scheme of the plasmid pGLG61_UOX (8.5 kb). The HpAOX promoter and UOX ORF with terminator region are shown as open boxes. The LEU2 gene H. polymorpha is shown as hatched box. Gene AmpR conferring resistance to ampicillin is shown as chequered boxes. The H. polymorpha truncated glyceraldehyde-3-phosphate dehydrogenase (GAP) promoter and the geneticin resistance gene (APH) are shown as grey boxes, and the tellomeric region (TEL188) as black box. Restriction sites: B, BamHI; K, KpnI. B: Visualization of UOX activity in situ in cells grown on mineral medium plates supplemented with glucose, incubated for 18 h, and then overlain with UOX reaction mixture with permeabilizing agent as described in Materials and Methods section.