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Figure 4 | BMC Biotechnology

Figure 4

From: Incorporation of albumin fusion proteins into fibrin clots in vitro and in vivo: comparison of different fusion motifs recognized by factor XIIIa

Figure 4

FXIIIa-mediated cross-linking of α 2 AP and XL5-HSA to fibrinogen. FXIII was pre-activated to fXIIIa by thrombin, the thrombin inactivated with FPRck, and the fXIIIa then combined with fibrinogen and α2AP (A) or XL5-HSA (B) in transglutamination reactions probed, after reducing SDS-polyacrylamide electrophoresis, with either (A) anti-α2AP or (B) anti-HSA antibodies. The position of pre-stained molecular mass markers is indicated to the left of the panels. Reactions containing only FXIII, only fibrinogen, or only α2AP are also shown.

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BMC Biotechnology

ISSN: 1472-6750

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