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Table 1 FP primers (no hair pin structure) and universal primers used in FPNI-PCR.

From: Fusion primer and nested integrated PCR (FPNI-PCR): a new high-efficiency strategy for rapid chromosome walking or flanking sequence cloning

Name

Primer sequence 5'-3'

Primer use

FP1:

GTAATACGACTCACTATAGGGCACGCGTGGT NTCGA STWTS GWGTT

1st PCR primer

FP2:

GTAATACGACTCACTATAGGGCACGCGTGGT NGTCG ASWGA NAWGAA

1st PCR primer

FP3:

GTAATACGACTCACTATAGGGCACGCGTGGT WGTGN AGWAN CANAGA

1st PCR primer

FP4:

GTAATACGACTCACTATAGGGCACGCGTGGT AGWGN AGWAN CAWAGG

1st PCR primer

FP5:

GTAATACGACTCACTATAGGGCACGCGTGGT NGTAW AASGT NTSCA A

1st PCR primer

FP6:

GTAATACGACTCACTATAGGGCACGCGTGGT NGACG ASWGA NAWGAC

1st PCR primer

FP7:

GTAATACGACTCACTATAGGGCACGCGTGGT NGACG ASWGA NAWGAA

1st PCR primer

FP8:

GTAATACGACTCACTATAGGGCACGCGTGGT GTNCG ASWCA NAWGTT

1st PCR primer

FP9:

GTAATACGACTCACTATAGGGCACGCGTGGT NCAGC TWSCT NTSCTT

1st PCR primer

FSP1:

GTAATACGACTCACTATAGGGC

2nd PCR primer

FSP2:

ACTATAGGGCACGCGTGGT

3rd PCR primer