Table 3 Quantitative analysis of total cleavage percentage P of 3×pen aRNA by Pen17zyme1B and Pen17zyme2
Molar ratio of 3×pen aRNA: Pen17zyme1B: Pen17zyme2 | Reaction time (hours) | 71-nt final product yield related to the initial 3×pen aRNA amount | 137-nt semi-product yield related to the initial 3×pen aRNA amount | P (Total cleavage percentage) |
|---|---|---|---|---|
1:1:1 | 17 | 30% | 15% | 45% |
1:10:1 | 17 | 39% | 16% | 55% |
1:10:2 | 17 | 42% | 15% | 57% |
1:10:1 | 40 | 70% | 22% | 92% |
1:10:10 | 40 | 77% | 23% | 100% |
1:10:10 | 72 | 90% | 10% | 100% |