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Table 3 Quantitative analysis of total cleavage percentage P of 3×pen aRNA by Pen17zyme1B and Pen17zyme2

From: DNAzyme-mediated recovery of small recombinant RNAs from a 5S rRNA-derived chimera expressed in Escherichia coli

Molar ratio of 3×pen aRNA: Pen17zyme1B: Pen17zyme2 Reaction time (hours) 71-nt final product yield related to the initial 3×pen aRNA amount 137-nt semi-product yield related to the initial 3×pen aRNA amount P (Total cleavage percentage)
1:1:1 17 30% 15% 45%
1:10:1 17 39% 16% 55%
1:10:2 17 42% 15% 57%
1:10:1 40 70% 22% 92%
1:10:10 40 77% 23% 100%
1:10:10 72 90% 10% 100%
  1. Cleavage reactions were performed under conditions described in legend to Figure 2 except for the reaction time and 3×pen aRNA: DNazymes molar ratio, which were as indicated above.