Figure 4

Separation of the products of 3× pen aRNA cleavage using streptavidin agarose beads. Collected fractions were analyzed on denaturing 8% PAGE. Lane1, LWM; Lane 2, 3×pen aRNA; Lane 3, Original cleavage reaction mixture; Lanes 4-5: Isolation of biotinylated DNAzymes, first and second elutions from the beads, respectively; Lane 6: Isolation of 71-nt excision product on the beads saturated with biotinylated complementary deoxyoligonucleotide, bioantiPEN; Lane 7: Recovery of bioantiPEN from the beads. Cleavage reaction was performed for 72 hours under conditions described in legend to Figure 2, with 3×pen aRNA: bioPen17zyme1B: bioPen17zyme2 molar ratio 1:10:10.