Figure 7

Specificity of RAb^DMvII1 by Flow cytometry. Flow cytometric analysis of surface expressed EGFRvIII. A) 1 × 10⁶ cells HC2 cells were stained with increasing concentration of RAb^DMvIII: 0.1 μg (purple), 1 μg (red) and 2 μg (green). The dot blot (left panel) and histogram in blue represents human-IgG1 isotype control. B) NIH3T3 and HC2 cells were mixed in a 1:1 ratio prior to staining with RAb^DMvIII revealing distinct identification of an EGFRvIII cell population. C) Overlay of flow cytometric plots of A431 (black) cells and HC2 cells (blue) stained with RAb^DMvIII, showing clear selectivity for EGFRvIII expression over EGFR expression. D) Freshly dissociated human GBM tumor stained with RAb^DMvIII shows the presence of surface EGFRvIII on 13% of cells (right panel). The dot blot (left panel) represents human-IgG1 isotype control.