Figure 2From: A simple fluorescence based assay for quantification of human immunodeficiency virus particle releaseTitration of eYFP labelled VLPs. Mixed particles were purified from the tissue culture supernatant of 293T cells transfected with an equimolar mixture of pCHIV and pCHIVeYFP by centrifugation through a sucrose cushion and their concentration determined by p24 ELISA. The relative eYFP fluorescence intensity of serial dilutions in DMEM/10% FCS was determined using a TECAN Saphire multi-well reader. Background fluorescence from the medium was subtracted and fluorescence intensities of the samples were plotted as a function of p24 CA concentration. The plot shows mean values and standard deviation from triplicate dilutions; the line represents a linear regression. The inset shows an expansion of the lower part of the same data set. The line represents a linear regression to the data points shown.Back to article page