Figure 3

PCR analysis for site-specific recombination and the presence of the phiC31 gene in the TR ₁ and BC ₁ generations. PCR reactions (a, c) with primers eand f(Fig. 1) or (b, d) with primers gand h(Fig. 1) on representative plant DNAs. a, b) retransformed TR₁- phiC31.22 lines. c, d) Back Crossed line BC₁-phiC31.22.3. Unlined numbers represent excision or recombinase only plants lines. Control lanes are B (blank, no DNA); E (excision, pN3-phiC31_exc); N (no excision, pN3-phiC31); P (recombinase, pCOXS3- phiC31).