Recent articles

View all articles

Most accessed articles

Most Accessed Articles: BMC Biotechnologyhttp://bmcbiotechnol.biomedcentral.comMost Accessed Articles: BMC BiotechnologyAn efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol plays an essential role in molecular biology and biochemistry. It has also been used in enzymology and protein science to generate proteins which are more tractable for biophysical techniques. The ...Thu, 04 Dec 2008 00:00:00 GMT Liu and James H Naismith2008-12-04T00:00:00ZFastCloning: a highly simplified, purification-free, sequence- and ligation-independent PCR cloning method a variety of methods and expensive kits are available, molecular cloning can be a time-consuming and frustrating process.Wed, 12 Oct 2011 00:00:00 GMT Li, Aiyun Wen, Benchang Shen, Jia Lu, Yao Huang and Yongchang Chang2011-10-12T00:00:00ZHigh level transient production of recombinant antibodies and antibody fusion proteins in HEK293 cells demand of monospecific high affinity binding reagents, particularly monoclonal antibodies, has been steadily increasing over the last years. Enhanced throughput of antibody generation has been addressed by...Wed, 26 Jun 2013 00:00:00 GMT Jäger, Konrad Büssow, Andreas Wagner, Susanne Weber, Michael Hust, André Frenzel and Thomas Schirrmann2013-06-26T00:00:00ZSimplified production and concentration of lentiviral vectors to achieve high transduction in primary human T cells vectors have emerged as efficient vehicles for transgene delivery in both dividing and non-dividing cells. A number of different modifications in vector design have increased biosafety and transgene...Tue, 12 Nov 2013 00:00:00 GMT P Cribbs, Alan Kennedy, Bernard Gregory and Fionula M Brennan2013-11-12T00:00:00ZSimple and efficient site-directed mutagenesis using two single-primer reactions in parallel to generate mutants for protein structure-function studies protein engineering, site-directed mutagenesis methods are used to generate DNA sequences with mutated codons, insertions or deletions. In a widely used method, mutations are generated by PCR using a pair o...Tue, 30 Jun 2009 00:00:00 GMT Edelheit, Aaron Hanukoglu and Israel Hanukoglu2009-06-30T00:00:00Z

View all articles

Aims and scope

BMC Biotechnology is an open access, peer-reviewed journal that considers articles on the manipulation of biological macromolecules or organisms for use in experimental procedures, cellular and tissue engineering or in the pharmaceutical, agricultural biotechnology and allied industries.

Section Editors

  • Joaquim M Cabral, Instituto Superior Tecnico
  • Dennis Claessen, Leiden University
  • Lluis Montoliu, Centro Nacional de Biotecnologia
  • Peter J Punt, TNO Microbiology & Systems Biology/Leiden University
  • Marko Radic, University of Tennessee
  • John J Rossi, Beckman Research Institute City of Hope
  • Igor Stagljar, University of Toronto
  • Neal C Stewart, University of Tennessee

Executive Editor

  • Dafne Solera, BioMed Central