Fig. 5
From: Analysis of the impact of pluronic acid on the thermal stability and infectivity of AAV6.2FF
Impact of 0.001% PF-68 on the in vivo transduction efficiency of AAV6.2FF. Six-week-old C57BL/6 female mice (n=7) were intranasally administered 1x1011 vg of AAV6.2FF-eGFP formulated in either PBS or PBS+0.001% PF-68 and 5 weeks later, lungs were harvested and analyzed for GFP expression by flow cytometry. A The percentage of GFP expressing live epithelial cells (n=7) in either buffer formulation compared to an untreated control using two-way ANOVA. * P ≤ 0.05, **** P ≤ 0.0001. B Analysis of the different live cell populations (7AAD-, CD45+/-, EpCAM+/-) that were transduced by AAV6.2FF-eGFP (n=4)