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Fig. 8 | BMC Biotechnology

Fig. 8

From: Rice yellow mottle virus is a suitable amplicon vector for an efficient production of an anti-leishmianiasis vaccine in Nicotiana benthamiana leaves

Fig. 8

RYMVMg1ΔP1ΔCP/CterPSA produces CterPSA protein under optimized conditions N. benthamiana leaves were infiltrated with RYMVMg1ΔP1ΔCP/CterPSA construct alone (RYMVMg1ΔP1ΔCP/CterPSA : empty vector 1:1 ratio) or with CP and P19 constructs either alone (RYMVMg1ΔP1ΔCP/CterPSA:CP or P19:empty vector 1:0,5:0,5 ratio) or together (RYMVMg1ΔP1ΔCP/CterPSA:CP:P19 1:2,5:5 ratio). Infiltrated areas were harvested at 12 dpi. The 35S::PSA construct in combination with the P19/P1/P0 RNA silencing suppressor cocktail was used as a control under the same conditions as described [7]. Total soluble proteins were prepared. Equal amounts of proteins (100 µg) were separated by SDS-PAGE and analyzed by immunoblotting using an anti-PSA antibody. Bands corresponding to PSA constructs and their corresponding sizes are indicated. Three independent experiments were performed. The results shown here are representative of the three experiments

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BMC Biotechnology

ISSN: 1472-6750

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