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Fig. 7 | BMC Biotechnology

Fig. 7

From: Rice yellow mottle virus is a suitable amplicon vector for an efficient production of an anti-leishmianiasis vaccine in Nicotiana benthamiana leaves

Fig. 7

Comparison of total RNA accumulation with polyA mRNA accumulation. Total RNA previously prepared from N. benthamiana leaves infiltrated with either RYMVMg1ΔP1ΔCP/CterPSA or 35S::PSA constructs was reverse transcribed using either oligodT or a mixture of oligodT and CterPSA specific primer (CterPSAspe). Three of the five individual plants were considerate. For both RYMVMg1ΔP1ΔCP/CterPSA A and 35S:PSA B samples, q-RT-PCR was performed using CterPSA-specific primers. Normalizations were performed using GAPDH as an internal housekeeping control. For each construct, expressions were evaluated relative to the initial RT oligodT condition. The three independent plants are represented by different colors. C- The ratio of total RNA to mRNA accumulation was compared between RYMVMg1ΔP1ΔCP/CterPSA and 35S::PSA samples. * Student t-test p=0,001

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BMC Biotechnology

ISSN: 1472-6750

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