Skip to main content
Fig. 6 | BMC Biotechnology

Fig. 6

From: Rice yellow mottle virus is a suitable amplicon vector for an efficient production of an anti-leishmianiasis vaccine in Nicotiana benthamiana leaves

Fig. 6

Accumulation of RYMVMg1ΔP1ΔCP/CterPSA antisense RNA. N. benthamiana leaves were infiltrated with RYMVMg1ΔP1ΔCP/CterPSA construct together with CP and P19 constructs under the optimal ratio of 1:5:2,5 amplicon:P19:CP. The35S::PSA construct in combination with the P19/P1/P0 RNA silencing suppressor cocktail was used as a control. Five individual plants, numbered from 1 to 5 were considered from both constructs. RNA was prepared from leaves infiltrated at 7 dpi. Reverse transcription was performed using either PSA primers hybridizing to the minus strand of the Cterm part of the PSA sequence (PSAfor) or oligodT primers. The primer used for reverse transcription is indicated in parentheses. PCR amplification was performed using PSA-specific primers or NbEF1-α primers. Negative (-) and positive (+) PCR controls were used

Back to article page

BMC Biotechnology

ISSN: 1472-6750

Contact us