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Fig. 4 | BMC Biotechnology

Fig. 4

From: Rice yellow mottle virus is a suitable amplicon vector for an efficient production of an anti-leishmianiasis vaccine in Nicotiana benthamiana leaves

Fig. 4

Optimal CP:P19 ratio for RYMVMg1ΔP1ΔCP/CterPSA RNA accumulation. N. benthamiana leaves were infiltrated with the RYMVMg1ΔP1ΔCP/CterPSA construct together with CP and P19 constructs. Four different amplicon:P19:CP ratios were considered. Infiltrated leaves were harvested at 5 dpi. Two independent experiments were performed. q-RT-PCR was performed after reverse transcription of RNA using a mixture of oligodT and CterPSA specific primers. PCR amplification was performed with CterPSA-specific primers. GAPDH was used as an internal housekeeping control. For each ratio, expressions were evaluated relative to the baseline ratio of 1:0,5:0,5. The two independent experiments are represented by different colors

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BMC Biotechnology

ISSN: 1472-6750

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