Method | Buffer | Components |
---|---|---|
Modified high-salt low-pH method | Buffer A | 1.25Â M NaCl, 0.25Â M Ascorbic acid, 10 mM Sodium metabisulfite, 50 mM Tris [tris (hydroxymethyl) aminomethane hydrochloride] -HCl (pH 8.0), 7 mM EDTA (ethylene diamine tetraacetic acid), 1% w/v PVP (polyvinylpyrrolidone)-40, 0.1% w/v BSA (bovine serum albumin), 1 mM DTT (dithiothreitol) |
Buffer B | 1.25Â M NaCl, 50 mM Tris-HCl (pH 8.0), 25 mM EDTA (pH 8.0), 1% w/v PVP-40, 0.1% w/v BSA, 1 mM DTT | |
Buffer C | 50 mM Tris-HCl, 25 mM EDTA (pH 8.0), 1.25Â M NaCl, 2.0% SDS (sodium dodecyl sulfate) | |
High-salt method | Buffer D | 1.25 M NaCl, 50 mM Tris-HCl (pH 8.0), 5 mM EDTA, 0.1% w/v BSA, 0.1% v/v β-mercaptoethanol |
NaOH low-salt method | Buffer E | 0.5 M NaOH, 7 mM EDTA, 1 g PVP-40, 0.2% v/v β-mercaptoethanol |
Buffer F | 0.62Â M NaCl, 50 mM Tris-HCl, 7 mM EDTA, 2Â g PVP-40, 1.5 mM DTT | |
Buffer G | 0.35Â M Sorbitol, 50 mM Tris-HCl, 25 mM EDTA |