Table 2 Primers list
Gene | Function | Primer sequence | Reference |
|---|---|---|---|
RNAIII | S. aureus QS gene | F: GCACTGAGTCCAAGGAAACTAACTCT R: AGCCATCCCAACTTAATAACCATGT | 1 |
rhlI | P. aeruginosa QS gene | F: TTCATCCTCCTTTAGTCTTCCC′ R: TTCCAGCGATTCAGAGAGC | 2 |
rhlR | P. aeruginosa QS gene | F: TGCATTTTATCGATCAGGGC R: CACTTCCTTTTCCAGGACG | 2 |
lasI | P. aeruginosa QS gene | F: TTTGGATCCTATACTCTCTGA R: ACGCAACTTGTGGATCCCGC | 12 |
lasR | P. aeruginosa QS gene | F: AAGTGGAAAATTGGAGTGGAG R: GTAGTTGCCGACGACGATGAAG | 2 |
algD | P. aeruginosa virulence gene (alginate production) | F: ATGCGAATCAGCATCTTTGGT R: CTACCAGCAGATGCCCTCGGC | 12 |
rpoD P. aeruginosa | P. aeruginosa housekeeping gene | F: GGGCTGTCTCGAATACGTTGA R: ACCTGCCGGAGGATATTTCC | 11 |
nucA S. aureus | S. aureus reference gene | F: AGCCAAGCCTTGACGAACTAAAGC R: GCGATTGATGGTGAT ACGGTT | 28 |