Fig. 2
The bacterial gene expression levels in treated and control groups in animal models. a: The expression level of RNAII, RNAIII of S. aureus and lasI/R, rhlI/R of P.aeruginosa co-infection groups. C1, C5, and C10 indicate the control groups on days 1, 5, and 10 after infection. T1, T5, and T10 indicate the treated groups in days 1, 5, and 10 after infection. b: The expression level of RNAII, RNAIII of S. aureus and lasI/R, rhlI/R of P.aeruginosa mono-infection groups. C1, C5, and C10 indicate the control groups in days 1, 5, and 10 after infection. T1, T5, and T10 indicate the treated groups on days 1, 5, and 10 after infection. c: The stereomicroscope images of P. aeruginosa isolates recovered from the treated group on co-infection. The rough edge of the colony indicated the up-regulation of Rhl QS system. d: The stereomicroscope images of P. aeruginosa isolates recovered from the control group on co-infection. The smooth edge of the colony indicated the down-regulation of Rhl QS system. e and f: The stereomicroscope images of P. aeruginosa isolates recovered from treated (c) and control (d) groups on mono-infection. An effect similar to co-infection was observed in the mono-infection group, as well. g and h: The TEM images of animal tissue co-infected with S. aureus and P. aeruginosa in treatment (g) and control (h) groups. The bacteria embedded in the biofilm structure were indicated by red arrows. The magnification of the images was 2 μm. i: The expression level of genes regulated biofilm formation in S. aureus and P. aeruginosa in co-infection groups (treatment and control). C1, C5, and C10 indicate the control groups in days 1, 5, and 10 after infection. T1, T5, and T10 indicate the treated groups on days 1, 5, and 10 after infection. j: The expression level of genes regulated biofilm formation in S. aureus and P. aeruginosa in mono-infection groups (treatment and control). C1, C5, and C10 indicate the control groups on days 1, 5, and 10 after infection. T1, T5, and T10 indicate the treated groups in days 1, 5, and 10 after infection. k: The biofilm formation capacity of S. aureus and P. aeruginosa in co- and mono-culture experiments on L-929 cell line in control and treatment groups. Each data set was analyzed using the two-way ANOVA, and the Holm-Sidak method for multiple comparisons. The data were presented as Mean + SEM. * p-value < 0.05; ** p-value < 0.01; *** p-value < 0.001; **** p-value < 0.0001