Fig. 1
Investigation of the effect of staphopain A on viability and biofilm disruption in cell line and animal model. a: The viable colony counts of S. aureus and P.aeruginosa on L-929 cell line in treatment and control groups. The recovered colonies from co- and mono-cultures were counted in the planktonic condition. b: The viable colony counts of S. aureus and P.aeruginosa on L-929 cell line in treatment and control groups. The recovered colonies from co- and mono-cultures were counted in the biofilm condition. c: The viable colonies recovered from the wound co-infected with S. aureus and P. aeruginosa during 10 days of treatment with staphopain A. It seems that the biofilm was disrupted during 5 days of treatment and the viable colonies were increased, then the killing effect of staphopain A led to a decrease in colony counts. d: The viable colonies recovered from the wound infected with S. aureus and P. aeruginosa (mono-infection group) during 10 days of treatment with staphopain A S. aureus and P. aeruginosa strains used in this study weakly formed biofilm and the higher initial counts seem to be due to weak single biofilms. e: The wound healing process during 10 days of experiment in co-infections groups of control and treatment. Panel e1 indicated the co-infection group during days 1 to 10 after treatment. As depicted, the wound drainage decreased, the wound diameter reduced significantly, and the infection healed successfully. While in the control group (row e2) the wound infection worsened during 10 days. f: The wound healing process during 10 days of experiment in groups infected with S. aureus. The wound healing process in the treatment group (row f1) improved successfully during 10 days in comparison to the control group (row f2). g: The wound healing process during 10 days of experiment in groups infected with P. aeruginosa. The wound healing process in the treatment group (row g1) improved successfully during 10 days in comparison to the control group (row g2). Each data set was analyzed using the two-way ANOVA, and the Holm-Sidak method for multiple comparisons. The data were presented as Mean + SEM. * p-value < 0.05; ** p-value < 0.01; *** p-value < 0.001; **** p-value < 0.0001