Fig. 2
From: A solution for highly efficient electroporation of primary cytotoxic T lymphocytes
Cell viability of mouse CTLs electroporated with pmax-GFP was increased using new recovery medium. A Representative flow cytometry analysis of cell viability of primary mouse CTLs after electroporation. Cells were transfected with pmax-GFP and kept to recover in the indicated recovery media for 12 hours at 32 °C. Viable cells were gated based on forward and side scatter. Cell viability was normalized to control (untransfected cells). Numbers in plots represent the percentage of viable cells in the gate. B Quantitative analysis of cell viability 12 hours post-electroporation using the indicated recovery media. C Representative flow cytometry analysis of transfection efficiency 12 hours post-electroporation. Transfection efficiency corresponds to the percentage of GFP-positive cells relative to the percentage of viable cells in each condition. Numbers in plots represent the percentage of GFP-positive cells in the gate. D Quantitative analysis of the transfection efficiency measured 12 hours after electroporation. Data represents mean ± standard error of the mean (SEM). N = 3, *p < 0.05, **p < 0.01