Table 3 Extraction recovery rate of the LC-MS/MS method for the determination of CML in human plasma
Lot number | Experimental data about CML recovery rate (peak area) | Experimental data about CEL recovery rate (peak area) | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
0.150 μmol/L | 0.450 μmol/L | 1.000 μmol/L | 0.150 μmol/L | 0.450 μmol/L | 1.000 μmol/L | |||||||
A | B | A | B | A | B | A | B | A | B | A | B | |
1 | 43,077 | 44,283 | 129,667 | 133,188 | 277,543 | 276,647 | 190,615 | 195,451 | 571,266 | 574,928 | 1,182,238 | 1,192,645 |
37,761 | 43,120 | 118,137 | 128,090 | 258,082 | 262,827 | 181,802 | 190,568 | 531,581 | 547,983 | 1,143,725 | 1,146,286 | |
37,892 | 45,765 | 117,655 | 132,063 | 277,598 | 268,551 | 189,690 | 189,743 | 548,393 | 570,955 | 1,169,465 | 1,207,275 | |
Mean | 39,576.67 | 44,389.33 | 121,819.67 | 131,113.67 | 271,074.33 | 269,341.67 | 187,369.00 | 191,920.67 | 550,413.33 | 564,622.00 | 1,165,142.67 | 1,182,068.67 |
SD | 3032.09 | 1325.70 | 6800.26 | 2678.31 | 11,251.72 | 6943.84 | 4843.30 | 3085.06 | 19,919.49 | 14,546.08 | 19,616.95 | 31,840.36 |
RSD/% | 8% | 3% | 6% | 2% | 4% | 3% | 3% | 2% | 4% | 3% | 2% | 3% |
%A = (A /B)*100% | 89.16 | 92.91 | 100.64 | 97.63 | 97.48 | 98.57 | ||||||
mean %A | 94.24 | 97.89 | ||||||||||